Anti-F-Spondin Antibody (B-3) is a mouse monoclonal IgG2b (kappa light chain) F-Spondin antibody provided at 200 µg/ml
raised against amino acids 508-807 mapping at the C-terminus of F-Spondin of human origin
Anti-F-Spondin Antibody (B-3) is recommended for detection of F-Spondin and mature Spondin-1 of mouse, rat and human origin by WB, IP, IF and ELISA
Anti-F-Spondin Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of F-Spondin (B-3): sc-390182.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
F-Spondin Antibody (B-3) is a high quality monoclonal F-Spondin antibody (also designated F-Spondin antibody) suitable for the detection of the F-Spondin protein of mouse, rat and human origin. F-Spondin Antibody (B-3) is available as both the non-conjugated anti-F-Spondin antibody form, as well as multiple conjugated forms of anti-F-Spondin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. F-Spondin, also designated Spondin-1 or vascular smooth muscle growth-promoting factor, is a member of the subgroup of the Thrombospondin type 1 class molecules. F-Spondin is a secreted, extracellular matrix-attached protein which patterns axonal trajectories by promoting adhesion and outgrowth of commissural axons, in addition to inhibiting outgrowth of motor axons. F-Spondin contains two conserved domains at the amino terminus, FS1 and FS2, which are regions of homology with Reelin and Mindin. Additionally, F-Spondin contains either six or four thrombospondin repeats (TSRs) at the carboxyl terminus, which are typical of class 2 TSRs. The F-Spondin gene is expressed in the nervous system, mainly at the embryonic floor plate and the hippocampus. F-Spondin may play a role in promoting axonal regeneration after nerve injury and in inflammatory processes in the nervous system.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended starting dilution for Western blot for sc-390182: F-Spondin (B-3) monoclonal antibody?
Asked by: Trav11
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol:
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
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