F-Spondin Antibody (B-3): sc-390182
- F-Spondin Antibody (B-3) is a mouse monoclonal IgG2b κ F-Spondin antibody provided at 200 µg/ml
- raised against amino acids 508-807 mapping at the C-terminus of F-Spondin of human origin
- F-Spondin Antibody (B-3) is recommended for detection of F-Spondin and mature Spondin-1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-F-Spondin Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for F-Spondin Antibody (B-3) for WB applications. These reagents are now offered in bundles with F-Spondin Antibody (B-3) (see ordering information below).
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F-Spondin Antibody (B-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects F-Spondin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). F-Spondin (B-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. F-Spondin, also known as Spondin-1 or vascular smooth muscle growth-promoting factor, is a crucial member of the Thrombospondin type 1 class molecules, functioning as a secreted, extracellular matrix-attached protein. F-Spondin plays a vital role in the nervous system by influencing axonal trajectories; promoting the adhesion and outgrowth of commissural axons while inhibiting the outgrowth of motor axons, which is essential for proper neural development and repair. F-Spondin contains two conserved domains at the amino terminus, FS1 and FS2, which share homology with Reelin and Mindin, and features either six or four thrombospondin repeats at the carboxyl terminus, characteristic of class 2 thrombospondin repeats. F-Spondin gene expression is primarily localized in the nervous system, particularly in the embryonic floor plate and the hippocampus, suggesting involvement in critical processes such as axonal regeneration following nerve injury and in various inflammatory responses within the nervous system.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
F-Spondin Antibody (B-3) References:
- F-spondin is a contact-repellent molecule for embryonic motor neurons. | Tzarfati-Majar, V., et al. 2001. Proc Natl Acad Sci U S A. 98: 4722-7. PMID: 11287656
- Plasmin-mediated release of the guidance molecule F-spondin from the extracellular matrix. | Tzarfaty-Majar, V., et al. 2001. J Biol Chem. 276: 28233-41. PMID: 11359777
- The neuronal class 2 TSR proteins F-spondin and Mindin: a small family with divergent biological activities. | Feinstein, Y. and Klar, A. 2004. Int J Biochem Cell Biol. 36: 975-80. PMID: 15094111
- VSGP/F-spondin: a new ovarian cancer marker. | Pyle-Chenault, RA., et al. 2005. Tumour Biol. 26: 245-57. PMID: 16103746
- Characterization of AmphiF-spondin reveals the modular evolution of chordate F-spondin genes. | Shimeld, SM. 1998. Mol Biol Evol. 15: 1218-23. PMID: 9729886
- Accumulation of F-spondin in injured peripheral nerve promotes the outgrowth of sensory axons. | Burstyn-Cohen, T., et al. 1998. J Neurosci. 18: 8875-85. PMID: 9786993
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
F-Spondin Antibody (B-3) | sc-390182 | 200 µg/ml | $322.00 | |||
F-Spondin Antibody (B-3): m-IgGκ BP-HRP Bundle | sc-523438 | 200 µg Ab, 40 µg BP | $361.00 | |||
F-Spondin Antibody (B-3): m-IgG2b BP-HRP Bundle | sc-549118 | 200 µg Ab; 10 µg BP | $361.00 | |||
F-Spondin Antibody (B-3) AC | sc-390182 AC | 500 µg/ml, 25% agarose | $424.00 | |||
F-Spondin Antibody (B-3) HRP | sc-390182 HRP | 200 µg/ml | $322.00 | |||
F-Spondin Antibody (B-3) FITC | sc-390182 FITC | 200 µg/ml | $336.00 | |||
F-Spondin Antibody (B-3) PE | sc-390182 PE | 200 µg/ml | $349.00 | |||
F-Spondin Antibody (B-3) Alexa Fluor® 488 | sc-390182 AF488 | 200 µg/ml | $364.00 | |||
F-Spondin Antibody (B-3) Alexa Fluor® 546 | sc-390182 AF546 | 200 µg/ml | $364.00 | |||
F-Spondin Antibody (B-3) Alexa Fluor® 594 | sc-390182 AF594 | 200 µg/ml | $364.00 | |||
F-Spondin Antibody (B-3) Alexa Fluor® 647 | sc-390182 AF647 | 200 µg/ml | $364.00 | |||
F-Spondin Antibody (B-3) Alexa Fluor® 680 | sc-390182 AF680 | 200 µg/ml | $364.00 | |||
F-Spondin Antibody (B-3) Alexa Fluor® 790 | sc-390182 AF790 | 200 µg/ml | $364.00 |