Ezrin Antibody (4i251): sc-71080

Ezrin Antibody (4i251) is a mouse monoclonal IgG1 antibody that detects Ezrin in mouse, rat, human, and bovine samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Ezrin is a crucial member of the ERM (Ezrin, Radixin, Moesin) protein family, which plays a vital role in linking the cytoskeleton to the plasma membrane, thereby facilitating cellular processes such as cell signaling, adhesion, and migration. Ezrin is predominantly localized beneath the plasma membrane, where Ezrin acts as a scaffold that organizes membrane proteins and cytoskeletal elements, which is essential for maintaining cell shape and structure. Notably, Ezrin is a significant substrate for various protein-tyrosine kinases, including the epidermal growth factor receptor, highlighting Ezrin′s involvement in signaling pathways that regulate cell growth and differentiation. Additionally, Ezrin functions as an anchoring protein for cAMP-dependent protein kinase, known as AKAP78, which underscores Ezrin′s role in spatially organizing signaling complexes within the cell. The distinct phosphorylation patterns exhibited by Ezrin, Moesin, and Radixin, despite their high homology, suggest that these proteins may have specialized functions in different cellular contexts, making anti-Ezrin antibody (4i251) an invaluable tool for researchers studying the dynamics of cell membrane interactions and cytoskeletal organization.

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Ezrin Antibody (4i251) References:

1. Ezrin, Radixin and Moesin: key regulators of membrane-cortex interactions and signaling.  |  Neisch, AL. and Fehon, RG. 2011. Curr Opin Cell Biol. 23: 377-82. PMID: 21592758
2. Ezrin Inhibition Up-regulates Stress Response Gene Expression.  |  Çelik, H., et al. 2016. J Biol Chem. 291: 13257-70. PMID: 27137931
3. Bidirectional Tumor-Promoting Activities of Macrophage Ezrin.  |  Khan, K., et al. 2020. Int J Mol Sci. 21: PMID: 33086476
4. Ezrin knockdown reduces procaterol-stimulated ciliary beating without morphological changes in mouse airway cilia.  |  Kawaguchi, K., et al. 2022. J Cell Sci. 135: PMID: 35132996
5. A biophysical perspective of the regulatory mechanisms of ezrin/radixin/moesin proteins.  |  Senju, Y. and Tsai, FC. 2022. Biophys Rev. 14: 199-208. PMID: 35340609
6. Expression of ezrin protein and phosphorylated ezrin in pelvic endometriotic lesions.  |  Peloggia, A., et al. 2022. Clinics (Sao Paulo). 77: 100074. PMID: 35793608
7. Ezrin and CD44 participate in the internalization process of Coxiella burnetii into non-phagocytic cells.  |  Distel, JS., et al. 2022. Biol Cell. 114: 237-253. PMID: 35851960
8. O-GlcNAcylation regulates phagocytosis by promoting Ezrin localization at the cell cortex.  |  Yang, S., et al. 2023. J Genet Genomics. 50: 486-496. PMID: 36796536
9. Contribution of radixin and ezrin to the maintenance of hepatocytes' excretory function in health and disease.  |  Dellbrügge, F., et al. 2023. Heliyon. 9: e21009. PMID: 37928027
10. Celastrol alleviates atopic dermatitis by regulating Ezrin-mediated mitochondrial fission and fusion.  |  Wang, D., et al. 2024. J Cell Mol Med. 28: e18375. PMID: 39039796

The preferred Ezrin antibody is Ezrin Antibody (3C12): sc-58758.