EYA4 Antibody (E-11): sc-393111
- EYA4 Antibody (E-11) is a mouse monoclonal IgG1 κ EYA4 antibody provided at 200 µg/ml
- raised against amino acids 153-276 mapping within an internal region of EYA4 of human origin
- EYA4 Antibody (E-11) is recommended for detection of EYA4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and bovine and porcine
- Anti-EYA4 Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for EYA4 Antibody (E-11) for WB and IHC(P) applications. These reagents are now offered in bundles with EYA4 Antibody (E-11) (see ordering information below).
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EYA4 Antibody (E-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects EYA4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-EYA4 antibody (E-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EYA4 protein, encoded by a gene located on chromosome 6q23, consists of 640 amino acids and is a member of the eyes absent family, which includes four distinct proteins. A notable feature of EYA4 is its highly conserved 271 amino acid domain at the carboxyl terminal, which plays a crucial role in function. This domain is essential for EYA4 interaction with other proteins such as SIX and DACH, as well as G-proteins, which are vital for regulating transcription during early embryonic development. Nuclear translocation of EYA4, facilitated by SIX, underscores importance in gene regulation. Mutations leading to premature stop codons in EYA4 have been linked to postlingual, progressive autosomal dominant hearing loss in humans, highlighting critical role in the mature organ of Corti. Additionally, EYA4 expression pattern suggests potential involvement in oculo-dento-digital syndrome, further emphasizing significance in developmental processes and relevance in various pathologies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
EYA4 Antibody (E-11) References:
- Cooperation of six and eya in activation of their target genes through nuclear translocation of Eya. | Ohto, H., et al. 1999. Mol Cell Biol. 19: 6815-24. PMID: 10490620
- Synergistic regulation of vertebrate muscle development by Dach2, Eya2, and Six1, homologs of genes required for Drosophila eye formation. | Heanue, TA., et al. 1999. Genes Dev. 13: 3231-43. PMID: 10617572
- The alpha subunits of Gz and Gi interact with the eyes absent transcription cofactor Eya2, preventing its interaction with the six class of homeodomain-containing proteins. | Fan, X., et al. 2000. J Biol Chem. 275: 32129-34. PMID: 10906137
- Mutations in the transcriptional activator EYA4 cause late-onset deafness at the DFNA10 locus. | Wayne, S., et al. 2001. Hum Mol Genet. 10: 195-200. PMID: 11159937
- EYA4, a novel vertebrate gene related to Drosophila eyes absent. | Borsani, G., et al. 1999. Hum Mol Genet. 8: 11-23. PMID: 9887327
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EYA4 Antibody (E-11) | sc-393111 | 200 µg/ml | $322.00 | |||
EYA4 Antibody (E-11): m-IgG Fc BP-HRP Bundle | sc-530357 | 200 µg Ab; 10 µg BP | $361.00 | |||
EYA4 Antibody (E-11): m-IgGκ BP-HRP Bundle | sc-523807 | 200 µg Ab, 40 µg BP | $361.00 | |||
EYA4 Antibody (E-11): m-IgG1 BP-HRP Bundle | sc-544010 | 200 µg Ab; 20 µg BP | $361.00 | |||
EYA4 Antibody (E-11) AC | sc-393111 AC | 500 µg/ml, 25% agarose | $424.00 | |||
EYA4 Antibody (E-11) HRP | sc-393111 HRP | 200 µg/ml | $322.00 | |||
EYA4 Antibody (E-11) FITC | sc-393111 FITC | 200 µg/ml | $336.00 | |||
EYA4 Antibody (E-11) PE | sc-393111 PE | 200 µg/ml | $349.00 | |||
EYA4 Antibody (E-11) Alexa Fluor® 488 | sc-393111 AF488 | 200 µg/ml | $364.00 | |||
EYA4 Antibody (E-11) Alexa Fluor® 546 | sc-393111 AF546 | 200 µg/ml | $364.00 | |||
EYA4 Antibody (E-11) Alexa Fluor® 594 | sc-393111 AF594 | 200 µg/ml | $364.00 | |||
EYA4 Antibody (E-11) Alexa Fluor® 647 | sc-393111 AF647 | 200 µg/ml | $364.00 | |||
EYA4 Antibody (E-11) Alexa Fluor® 680 | sc-393111 AF680 | 200 µg/ml | $364.00 | |||
EYA4 Antibody (E-11) Alexa Fluor® 790 | sc-393111 AF790 | 200 µg/ml | $364.00 |