Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Ets-2 CRISPR/Cas9 KO Plasmid (m): sc-423871
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ets-2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Ets-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ets-2 Antibody (E-5): sc-365666
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ets-2 CRISPR/Cas9 KO Plasmid (m) | sc-423871 | 20 µg | $397.00 |
Overview
Mouse Ets2 encodes the ETS family transcription factor Ets-2, a DNA-binding regulator that modulates gene expression programs controlling proliferation, differentiation, survival, and extracellular matrix remodeling. Ets-2 integrates signals downstream of MAPK/ERK and related kinase cascades, enabling stimulus-dependent transcriptional responses that shape immune cell activation, developmental processes, and tissue homeostasis. Dysregulated ETS2 activity has been linked to altered inflammatory gene expression, aberrant stromal and epithelial behavior, and oncogenic transcriptional networks in multiple model systems. As a nodal transcriptional regulator, Ets-2 is commonly studied for its roles in transcriptional control of cytokines, matrix metalloproteinases, and cell-cycle-associated genes.
Ets-2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ets2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ets2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ets2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ets-2 protein expression.
This CRISPR knockout system enables efficient generation of Ets2-deficient cell models for investigation of Ets-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ets2 exon(s) critical for Ets-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ets2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Ets-2 CRISPR/Cas9 KO Plasmid (m) and Ets-2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ets2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Ets-2 HDR Plasmid (m) and Ets-2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ets2 homology arms to support homology-directed repair at defined Ets2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.