Estrogen Receptor alpha Antibody (D-12) recommended for detection of Estrogen Receptor alpha of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Estrogen Receptor alpha Antibody (D-12): sc-8005

Estrogen Receptor alpha Antibody (D-12) is rated 3.8 out of 5 by 20.
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Datasheets

    • Estrogen Receptor alpha Antibody (D-12) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 2-185 mapping at the N-terminus of Estrogen Receptor alpha of human origin
    • Anti-Estrogen Receptor alpha Antibody (D-12) is recommended for detection of Estrogen Receptor alpha of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Estrogen Receptor alpha Antibody (D-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to TRITC for IF, IHC(P) and FCM
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-8005 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Estrogen Receptor alpha (D-12): sc-8005.
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Hi, I want to do IHC for formalin fixed rat mammary gland, does this antibody work for rat tissues by IHC?

Asked by: Yanrong
Thank you for your question. Yes, this antibody is recommended for IHC(P) in mouse, rat and human tissues.
Answered by: Technical Support
Date published: 2019-08-17

Can we use this antibody for IF and IHC staining in paraffin-embedded mouse tissues?

Asked by: YuHsin
Thank you for your question. ERα Antibody (D-12): sc-8005 is recommended for detection of ERα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA, so using it for IF or IHC(P) staining should work well. For published data with this antibody in murine tissues and IHC(P) please see PMID 19332863 or PMID 22992387.
Answered by: Tech Support Europe
Date published: 2019-07-03

Can we use this antibody in rat brain samples for W.B. Immunoflourescence in frozen sections?

Asked by: Rukmani p
Thank you for your question. This mouse monoclonal antibody, ERα (D-12): sc-8005, is suitable for Western blotting (WB) and immunoflourescence (IF) in frozen tissues of rat origin. We have not tested this antibody specifically in rat brain tissue samples. Please refer to the data on our website for our recommended positive controls to include when testing this antibody in your samples.
Answered by: Technical Support
Date published: 2017-08-03

Can I use this antibody for immunoprecipitation of ER alpha from mouse brain?

Asked by: Victor86
Thank you for your question. This mouse monoclonal antibody, ERα (D-12): sc-8005, can be used for immunoprecipitation (IP) in mouse samples. We are not aware of any specific publications citing use of the antibody to IP ER alpha from brain tissue samples, but it has been successfully used for IP in both human and mouse cell lines.
Answered by: Technical Support
Date published: 2017-05-18

I need to use an antibody to detect the protein in samples of canineorigin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 3
Date published: 2016-12-21

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to Fluorescein (sc-8005 FITC) and TRITC (sc-8005 TRITC). Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-15

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-15
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Rated 3 out of 5 by from good antibody good antibody for western blotting and also immunofluorescence
Date published: 2019-01-21
Rated 2 out of 5 by from Unsure of molecular weight in Western blot A very faint band showed up at the correct molecular weight. A brighter band showed up at the incorrect molecular weight. Western Blot (1:100) in rat white adipose tissue.
Date published: 2018-09-12
Rated 4 out of 5 by from Good antibody I tried it on cultured human cells on IF (1:100, 1:200), both concentration worked.
Date published: 2018-04-07
Rated 5 out of 5 by from Great I had great signal for ICC on Human breast cancer cell lines.
Date published: 2018-02-27
Rated 2 out of 5 by from Ishikawa細胞ではいまひとつです。 石川細胞のタンパクを10μg applyしました。東洋紡のCan get signalシリーズを用いましたが、非特異的なバンドのほうが濃くでてします。
Date published: 2018-02-23
Rated 3 out of 5 by from Not quite suitable for wb of rat protein 1:200 diluted for wb of rat heart protein,the band is a little weak,i do not think it qutite suitable for wb of rat heart protein
Date published: 2018-01-24
Rated 1 out of 5 by from D-12抗体の使用感 ラットの培養細胞株およびMCF7抽出液を使ったウエスタンブロットを行ったが、目的位置にバンドが検出できなかった。
Date published: 2018-01-17
Rated 5 out of 5 by from Excellent by IF Staining is beautiful in mouse brain after antigen retrieval
Date published: 2017-12-01
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