ESET Antibody (G-4): sc-271488

ESET Antibody (G-4) is a mouse monoclonal IgG2b antibody that detects ESET in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). ESET, also known as Histone H3-K9 methyltransferase 4 or SET domain bifurcated 1, plays a crucial role in the regulation of gene expression by modifying chromatin structure through methyltransferase activity. Specifically, ESET is responsible for methylating Lys-9 of histone H3, a modification that serves as a signal for epigenetic transcriptional repression. This process is vital for maintaining cellular identity and regulating developmental pathways, as aberrations in ESET function can lead to misregulation of gene expression and contribute to various diseases, including cancer. The gene encoding ESET, SETDB1, is located on chromosome 1q21, and mutations in this gene can disrupt methyltransferase activity, highlighting ESET′s importance in epigenetic regulation. ESET is predominantly expressed in the testis, indicating a potential role in germ cell development and function. Anti-ESET antibody (G-4) serves as an invaluable tool for researchers studying epigenetic mechanisms and their implications in health and disease.

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ESET Antibody (G-4) References:

1. Molecular cloning of ESET, a novel histone H3-specific methyltransferase that interacts with ERG transcription factor.  |  Yang, L., et al. 2002. Oncogene. 21: 148-52. PMID: 11791185
2. Genomic structure and expression of the mouse ESET gene encoding an ERG-associated histone methyltransferase with a SET domain.  |  Blackburn, ML., et al. 2003. Biochim Biophys Acta. 1629: 8-14. PMID: 14522075
3. mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression.  |  Wang, H., et al. 2003. Mol Cell. 12: 475-87. PMID: 14536086
4. Histone H3-K9 methyltransferase ESET is essential for early development.  |  Dodge, JE., et al. 2004. Mol Cell Biol. 24: 2478-86. PMID: 14993285
5. Eset partners with Oct4 to restrict extraembryonic trophoblast lineage potential in embryonic stem cells.  |  Yuan, P., et al. 2009. Genes Dev. 23: 2507-20. PMID: 19884257
6. ESET histone methyltransferase is essential to hypertrophic differentiation of growth plate chondrocytes and formation of epiphyseal plates.  |  Yang, L., et al. 2013. Dev Biol. 380: 99-110. PMID: 23652029
7. Mesenchyme-specific knockout of ESET histone methyltransferase causes ectopic hypertrophy and terminal differentiation of articular chondrocytes.  |  Lawson, KA., et al. 2013. J Biol Chem. 288: 32119-32125. PMID: 24056368
8. In silico probing and biological evaluation of SETDB1/ESET-targeted novel compounds that reduce tri-methylated histone H3K9 (H3K9me3) level.  |  Park, I., et al. 2017. J Comput Aided Mol Des. 31: 877-889. PMID: 28879500
9. Smad3-mediated recruitment of the methyltransferase SETDB1/ESET controls Snail1 expression and epithelial-mesenchymal transition.  |  Du, D., et al. 2018. EMBO Rep. 19: 135-155. PMID: 29233829
10. Generation and characterization of mice with mesenchyme-specific deletion of the entire ESET histone methyltransferase protein.  |  Yang, L., et al. 2018. Genesis. 56: PMID: 29282851

The preferred ESET antibody is ESET Antibody (A-1): sc-166621.