ERp5 Antibody (G-5): sc-365260
- ERp5 Antibody (G-5) is a mouse monoclonal IgG2b κ ERp5 antibody, cited in 7 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 15-45 near the N-terminus of ERp5 of human origin
- ERp5 Antibody (G-5) is recommended for detection of ERp5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, bovine and porcine
- Anti-ERp5 Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ERp5 Antibody (G-5) for WB and IHC(P) applications. These reagents are now offered in bundles with ERp5 Antibody (G-5) (see ordering information below).
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ERp5 Antibody (G-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects ERp5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-ERp5 antibody (G-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ERp5, also known as PDIA6 or TXNDC7, is a 440 amino acid protein characterized by the presence of two thioredoxin domains, which are crucial for function as a protein disulfide isomerase. ERp5 is primarily localized in the endoplasmic reticulum and melanosomes, where ERp5 plays a vital role in catalyzing the rearrangement of disulfide bonds in various proteins, a process essential for proper protein folding and stability. ERp5′s ability to reduce disulfide bonds is particularly important in tumor biology, as ERp5 can release MICA from tumor cells, thereby modulating immune responses and potentially influencing tumor expansion. Multiple isoforms of ERp5 exist due to alternative splicing, highlighting the complexity of regulation and function, making ERp5 a significant target for research in cancer and cellular stress responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ERp5 Antibody (G-5) References:
- Functional analysis of human P5, a protein disulfide isomerase homologue. | Kikuchi, M., et al. 2002. J Biochem. 132: 451-5. PMID: 12204115
- A protein disulfide isomerase expressed in the embryonic midline is required for left/right asymmetries. | Hoshijima, K., et al. 2002. Genes Dev. 16: 2518-29. PMID: 12368263
- The gene for a novel protein, a member of the protein disulphide isomerase/form I phosphoinositide-specific phospholipase C family, is amplified in hydroxyurea-resistant cells. | Chaudhuri, MM., et al. 1992. Biochem J. 281 (Pt 3): 645-50. PMID: 1311171
- A role for the thiol isomerase protein ERP5 in platelet function. | Jordan, PA., et al. 2005. Blood. 105: 1500-7. PMID: 15466936
- Disulphide-isomerase-enabled shedding of tumour-associated NKG2D ligands. | Kaiser, BK., et al. 2007. Nature. 447: 482-6. PMID: 17495932
- Cloning and sequencing of the cDNA encoding human P5. | Hayano, T. and Kikuchi, M. 1995. Gene. 164: 377-8. PMID: 7590364
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ERp5 Antibody (G-5) | sc-365260 | 200 µg/ml | $322.00 | |||
ERp5 Antibody (G-5): m-IgGκ BP-HRP Bundle | sc-522184 | 200 µg Ab, 40 µg BP | $361.00 | |||
ERp5 Antibody (G-5): m-IgG2b BP-HRP Bundle | sc-549007 | 200 µg Ab; 10 µg BP | $361.00 | |||
ERp5 Antibody (G-5) AC | sc-365260 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ERp5 Antibody (G-5) HRP | sc-365260 HRP | 200 µg/ml | $322.00 | |||
ERp5 Antibody (G-5) FITC | sc-365260 FITC | 200 µg/ml | $336.00 | |||
ERp5 Antibody (G-5) PE | sc-365260 PE | 200 µg/ml | $349.00 | |||
ERp5 Antibody (G-5) Alexa Fluor® 488 | sc-365260 AF488 | 200 µg/ml | $364.00 | |||
ERp5 Antibody (G-5) Alexa Fluor® 546 | sc-365260 AF546 | 200 µg/ml | $364.00 | |||
ERp5 Antibody (G-5) Alexa Fluor® 594 | sc-365260 AF594 | 200 µg/ml | $364.00 | |||
ERp5 Antibody (G-5) Alexa Fluor® 647 | sc-365260 AF647 | 200 µg/ml | $364.00 | |||
ERp5 Antibody (G-5) Alexa Fluor® 680 | sc-365260 AF680 | 200 µg/ml | $364.00 | |||
ERp5 Antibody (G-5) Alexa Fluor® 790 | sc-365260 AF790 | 200 µg/ml | $364.00 | |||
ERp5 (G-5) Neutralizing Peptide | sc-365260 P | 100 µg/0.5 ml | $69.00 |