Ero1-Lα Antibody (YW-8): sc-100805

Ero1-Lα Antibody (YW-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects Ero1-Lα in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Ero1-Lα antibody (YW-8) is available in a non-conjugated form, allowing for versatile experimental use. Ero1-Lα, also known as endoplasmic oxidoreductin-1-like or oxidoreductin-1-Lα, plays a critical role as an oxidoreductase in the endoplasmic reticulum, where Ero1-Lα facilitates the formation of disulfide bonds in proteins, a vital process for proper protein folding and function. Ero1-Lα is particularly important for the maturation of immunoglobulins, which are essential components of the immune response. Ero1-Lα interacts with protein disulfide isomerase (PDI) to generate disulfide bonds, thereby ensuring that proteins achieve their correct three-dimensional structures. Additionally, Ero1-Lα is involved in the regulation of adiponectin secretion from adipose tissue, influencing metabolic processes, and Ero1-Lα modulates the activities of peroxisome proliferator-activated receptor gamma and SIRT1, which are crucial for metabolic homeostasis. Ero1-Lα expression is stimulated under hypoxic conditions, indicating regulation by the hypoxia-inducible transcription factor pathway, and Ero1-Lα is found at low levels throughout various tissues, with significantly higher expression in the upper digestive tract and esophagus. Ero1-Lα can function both as a monomer and as a homodimer, adding to functional versatility in cellular processes.

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Ero1-Lα Antibody (YW-8) References:

1. ERO1-L, a human protein that favors disulfide bond formation in the endoplasmic reticulum.  |  Cabibbo, A., et al. 2000. J Biol Chem. 275: 4827-33. PMID: 10671517
2. Pathways for protein disulphide bond formation.  |  Frand, AR., et al. 2000. Trends Cell Biol. 10: 203-10. PMID: 10754564
3. The CXXCXXC motif determines the folding, structure and stability of human Ero1-Lalpha.  |  Benham, AM., et al. 2000. EMBO J. 19: 4493-502. PMID: 10970843
4. The cellular oxygen tension regulates expression of the endoplasmic oxidoreductase ERO1-Lalpha.  |  Gess, B., et al. 2003. Eur J Biochem. 270: 2228-35. PMID: 12752442
5. Two conserved cysteine triads in human Ero1alpha cooperate for efficient disulfide bond formation in the endoplasmic reticulum.  |  Bertoli, G., et al. 2004. J Biol Chem. 279: 30047-52. PMID: 15136577
6. Dynamic retention of Ero1alpha and Ero1beta in the endoplasmic reticulum by interactions with PDI and ERp44.  |  Otsu, M., et al. 2006. Antioxid Redox Signal. 8: 274-82. PMID: 16677073
7. Intracellular catalysis of disulfide bond formation by the human sulfhydryl oxidase, QSOX1.  |  Chakravarthi, S., et al. 2007. Biochem J. 404: 403-11. PMID: 17331072
8. Secretion of the adipocyte-specific secretory protein adiponectin critically depends on thiol-mediated protein retention.  |  Wang, ZV., et al. 2007. Mol Cell Biol. 27: 3716-31. PMID: 17353260
9. Adiponectin secretion is regulated by SIRT1 and the endoplasmic reticulum oxidoreductase Ero1-L alpha.  |  Qiang, L., et al. 2007. Mol Cell Biol. 27: 4698-707. PMID: 17452443
10. Reconstitution of human Ero1-Lalpha/protein-disulfide isomerase oxidative folding pathway in vitro. Position-dependent differences in role between the a and a' domains of protein-disulfide isomerase.  |  Wang, L., et al. 2009. J Biol Chem. 284: 199-206. PMID: 19001419
11. A CHO cell line engineered to express XBP1 and ERO1-Lα has increased levels of transient protein expression.  |  Cain, K., et al. 2013. Biotechnol Prog. 29: 697-706. PMID: 23335490
12. Endoplasmic reticulum resident oxidase ERO1-Lalpha promotes hepatocellular carcinoma metastasis and angiogenesis through the S1PR1/STAT3/VEGF-A pathway.  |  Yang, S., et al. 2018. Cell Death Dis. 9: 1105. PMID: 30377291

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ero1-Lα Antibody (YW-8) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-Ero1-Lα Antibody (YW-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ero1-Lα Antibody (YW-8) Specifications

Alternate Product Name: Ero1-Lα

Product Clone Name: YW-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 90-179

Epitope Target: Ero1-Lα

Epitope Species: human

Product Reactivity/Detection: Ero1-Lα

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for Ero1-Lα Antibody (YW-8): N/A

The preferred Ero1-Lα antibody is Ero1-Lα Antibody (D-7): sc-365526.