ERK 2 Antibody (6G11): sc-81458

ERK 2 Antibody (6G11) is a mouse monoclonal IgG1 antibody raised against the C-terminus of ERK2 (Extracellular Signal-Regulated Kinase 2), also known as MAPK1 or p42 MAPK, of human origin. ERK2 is a crucial serine/threonine kinase within the Mitogen-Activated Protein Kinase (MAPK) signaling pathways, playing a vital role in transducing extracellular signals into intracellular responses that govern cell proliferation, differentiation, transcription regulation, and development. Structurally, ERK2 consists of an N-terminal lobe and a C-terminal lobe forming the typical kinase fold, with an ATP-binding site located between them. The activation loop contains the conserved Threonine-Glutamate-Tyrosine (TEY) motif, whose phosphorylation by upstream kinases like MEK1/2 is essential for ERK2 activation. This phosphorylation induces conformational changes that enhance catalytic activity, promote dimerization, and facilitate nuclear translocation, a critical step for phosphorylating nuclear substrates involved in gene expression. The C-terminus, targeted by ERK2 (6G11) monoclonal antibody, is important for protein-protein interactions and proper cellular localization, making it a significant region for studying ERK2 function. Anti-ERK2 antibody (6G11) is reactive with mouse, rat, human, and canine ERK2 proteins and is recommended for use in western blotting (WB) and immunoprecipitation (IP) applications. By targeting the C-terminal epitope, ERK2 (6G11) monoclonal antibody enables researchers to investigate the structural and functional aspects of ERK2 within the MAPK/ERK signaling pathway, providing insights into signal transduction mechanisms and their implications in various physiological and pathological processes.

The preferred p-ERK antibody is p-ERK Antibody (E-4): sc-7383.