ERK 1/2 Antibody (MK1) recommended for detection of ERK 1 and ERK 2 of mouse, rat, human and canine origin by WB, IP and IF

ERK 1/2 Antibody (MK1): sc-135900

ERK 1/2 Antibody (MK1) is rated 4.4 out of 5 by 14.
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Datasheets

    • ERK 1/2 Antibody (MK1) is a mouse monoclonal mouse IgG1 κ (kappa light chain) provided at 50 µg/0.5 ml
    • raised against amino acids 325-345 of ERK 1 of rat origin
    • recommended for detection of ERK 1 and ERK 2 of mouse, rat, human and canine origin by WB, IP and IF
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See ERK 2 (D-2): sc-1647 for ERK 1/2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ERK 1/2 (MK1): sc-135900.

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Anonymous
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2016-12-22

Will this ERK1/2 antibody detect the protein in bovine samples?

Asked by: Anonymous
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2016-12-22

Can ERK2 (SC-135900) be used as a loading control for western blot lysates?

Asked by: Eldhose
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Rated 5 out of 5 by from Excellent antibody for WBs I have used this antibody at 1:1000 dilution for western blotting using a number of human cell lines and obtained a very strong signal. Extremely pleased with this antibody.
Date published: 2019-06-15
Rated 1 out of 5 by from No signal in In-Cell Western This antibody did not work for my In-Cell Western assays in HEK293. I use 4% paraformaldehyde fixation and methanol permeabilization, and I don't know if it could work with a different protocol. I would not purchase this antibody for any immunocytochemistry approaches.
Date published: 2018-05-07
Rated 5 out of 5 by from good result This antibody can be used to publish using the antibody in WB and IHC in mouse neuron.
Date published: 2017-05-28
Rated 5 out of 5 by from good ERK 1/2 antibody worked well in the rat cerebrum tissue for WB analysis
Date published: 2017-05-13
Rated 5 out of 5 by from Works very well It was used for WB on rat cerebrum tissue extract., which shows good quality and got bands very clearly.
Date published: 2017-04-24
Rated 5 out of 5 by from positive band Dilution detection 1:1000. It shows the positive band, I am satisfied with the result.
Date published: 2017-02-26
Rated 3 out of 5 by from Low sensitivity for WB This ab has good specificity, but relatively low sensitivity for WB with ERK 1/2 from human, mouse and rat.
Date published: 2017-01-30
Rated 4 out of 5 by from band is clear Western work well in human protein samples.Do not detect the antibody in IHC of IF
Date published: 2016-12-29
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