ERH Antibody (C-6): sc-373906

ERH Antibody (C-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects ERH protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ERH antibody (C-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ERH protein, also known as enhancer of rudimentary homolog or DROER, is a 104 amino acid transcriptional coregulator that is ubiquitously expressed and highly conserved across eukaryotic species. One of the critical aspects of ERH is its role in regulating the stability of the HNF-1/DNA complex, which is essential for the expression of various genes in the liver. By repressing the coactivator PCBD, ERH disrupts the interaction between HNF-1 and DNA, thereby influencing the transcriptional activity of genes involved in liver function and metabolism. The structure of ERH is notable for its single domain, which comprises three alpha-helices and four beta-strands, along with a long, flexible loop that is highly conserved, indicating potential importance in functional dynamics. Additionally, ERH contains two phosphorylation sites for casein kinase II, which may affect dimerization ability, further underscoring the significance of post-translational modifications in modulating activity.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ERH Antibody (C-6) References:

1. ERH (enhancer of rudimentary homologue), a conserved factor identical between frog and human, is a transcriptional repressor.  |  Pogge von Strandmann, E., et al. 2001. Biol Chem. 382: 1379-85. PMID: 11688721
2. Structure of the conserved transcriptional repressor enhancer of rudimentary homolog.  |  Wan, C., et al. 2005. Biochemistry. 44: 5017-23. PMID: 15794639
3. Crystal structure of an enhancer of rudimentary homolog (ERH) at 2.1 Angstroms resolution.  |  Arai, R., et al. 2005. Protein Sci. 14: 1888-93. PMID: 15937287
4. A 1.55 A resolution X-ray crystal structure of HEF2/ERH and insights into its transcriptional and cell-cycle interaction networks.  |  Jin, T., et al. 2007. Proteins. 68: 427-37. PMID: 17444515
5. Ciz1, a p21 cip1/Waf1-interacting zinc finger protein and DNA replication factor, is a novel molecular partner for human enhancer of rudimentary homolog.  |  Lukasik, A., et al. 2008. FEBS J. 275: 332-40. PMID: 18081865
6. The bifunctional protein DCoH modulates interactions of the homeodomain transcription factor HNF1 with nucleic acids.  |  Rhee, KH., et al. 1997. J Mol Biol. 265: 20-9. PMID: 8995521

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ERH Antibody (C-6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-ERH Antibody (C-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ERH Antibody (C-6) Specifications

Alternate Product Name: ERH

Product Clone Name: C-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-104

Epitope Target: ERH

Epitope Species: human

Product Reactivity/Detection: ERH

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for ERH Antibody (C-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin