ERGIC-53 Antibody (G-11): sc-398685
- ERGIC-53 Antibody (G-11) is a mouse monoclonal mouse IgM κ κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 68-96 near the N-terminus of ERGIC-53 of human origin
- recommended for detection of ERGIC-53 of mouse, rat and human origin by WB, IP, IF and ELISA
- See ERGIC-53 (F-3): sc-398777 for ERGIC-53 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ERGIC-53 Antibody (G-11). This work is in progress.
ERGIC-53 Antibody (G-11) is a mouse monoclonal IgM antibody that detects ERGIC-53 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ERGIC-53, also known as LMAN1, plays a crucial role in intracellular transport of glycoproteins, specifically those with high mannose-type glycans, from the endoplasmic reticulum to the ER-Golgi intermediate compartment. ERGIC-53 is essential for proper sorting and recycling of glycoproteins and glycolipids, ensuring these molecules reach correct destinations within the cell. Notably, mutations or null expression of ERGIC-53 can lead to a rare autosomal recessive bleeding disorder characterized by a combined deficiency of coagulation factors V and VIII, highlighting ERGIC-53′s importance in maintaining hemostasis. Anti-ERGIC-53 antibody (G-11) is an invaluable tool for researchers studying mechanisms of glycoprotein transport and implications of ERGIC-53 in various diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ERGIC-53 Antibody (G-11) References:
- The cargo receptor ERGIC-53 is a target of the unfolded protein response. | Nyfeler, B., et al. 2003. Biochem Biophys Res Commun. 304: 599-604. PMID: 12727195
- pH-induced conversion of the transport lectin ERGIC-53 triggers glycoprotein release. | Appenzeller-Herzog, C., et al. 2004. J Biol Chem. 279: 12943-50. PMID: 14718532
- Heat shock induces preferential translation of ERGIC-53 and affects its recycling pathway. | Spatuzza, C., et al. 2004. J Biol Chem. 279: 42535-44. PMID: 15292203
- Regulation of ERGIC-53 gene transcription in response to endoplasmic reticulum stress. | Renna, M., et al. 2007. J Biol Chem. 282: 22499-512. PMID: 17535801
- The cargo receptors Surf4, endoplasmic reticulum-Golgi intermediate compartment (ERGIC)-53, and p25 are required to maintain the architecture of ERGIC and Golgi. | Mitrovic, S., et al. 2008. Mol Biol Cell. 19: 1976-90. PMID: 18287528
- The intracellular cargo receptor ERGIC-53 is required for the production of infectious arenavirus, coronavirus, and filovirus particles. | Klaus, JP., et al. 2013. Cell Host Microbe. 14: 522-34. PMID: 24237698
- Structural basis for disparate sugar-binding specificities in the homologous cargo receptors ERGIC-53 and VIP36. | Satoh, T., et al. 2014. PLoS One. 9: e87963. PMID: 24498414
- Crystallographic snapshots of the EF-hand protein MCFD2 complexed with the intracellular lectin ERGIC-53 involved in glycoprotein transport. | Satoh, T., et al. 2020. Acta Crystallogr F Struct Biol Commun. 76: 216-221. PMID: 32356523
- A virtuous cycle operated by ERp44 and ERGIC-53 guarantees proteostasis in the early secretory compartment. | Tempio, T., et al. 2021. iScience. 24: 102244. PMID: 33763635
- ERGIC-53, a membrane protein of the endoplasmic reticulum-Golgi intermediate compartment, is identical to MR60, an intracellular mannose-specific lectin of myelomonocytic cells. | Arar, C., et al. 1995. J Biol Chem. 270: 3551-3. PMID: 7876089
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ERGIC-53 Antibody (G-11) | sc-398685 | 200 µg/ml | $316.00 | |||
ERGIC-53 (G-11) Neutralizing Peptide | sc-398685 P | 100 µg/0.5 ml | $68.00 |