ERGIC-53 Antibody (B-4): sc-398893

ERGIC-53 Antibody (B-4) is a mouse monoclonal IgG3 antibody that detects ERGIC-53 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ERGIC-53, also known as LMAN1, plays a crucial role in intracellular transport of glycoproteins, specifically those with high mannose-type glycans, from the endoplasmic reticulum to the ER-Golgi intermediate compartment. ERGIC-53 is essential for proper sorting and recycling of glycoproteins and glycolipids, ensuring they reach correct destinations within the cell. Notably, mutations or null expression of ERGIC-53 can lead to a rare autosomal recessive bleeding disorder characterized by a combined deficiency of coagulation factors V and VIII, highlighting ERGIC-53′s importance in maintaining hemostasis. Anti-ERGIC-53 antibody (B-4) is an invaluable tool for researchers studying mechanisms of glycoprotein transport and implications of ERGIC-53 in various diseases.

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ERGIC-53 Antibody (B-4) References:

1. Sequence and expression of the monkey homologue of the ER-golgi intermediate compartment lectin, ERGIC-53.  |  Sarnataro, S., et al. 1999. Biochim Biophys Acta. 1447: 334-40. PMID: 10542336
2. ER export of ERGIC-53 is controlled by cooperation of targeting determinants in all three of its domains.  |  Nufer, O., et al. 2003. J Cell Sci. 116: 4429-40. PMID: 13130098
3. The crystal structure of the carbohydrate-recognition domain of the glycoprotein sorting receptor p58/ERGIC-53 reveals an unpredicted metal-binding site and conformational changes associated with calcium ion binding.  |  Velloso, LM., et al. 2003. J Mol Biol. 334: 845-51. PMID: 14643651
4. ER storage diseases: a role for ERGIC-53 in controlling the formation and shape of Russell bodies.  |  Mattioli, L., et al. 2006. J Cell Sci. 119: 2532-41. PMID: 16735443
5. N-glycosylation of human nicastrin is required for interaction with the lectins from the secretory pathway calnexin and ERGIC-53.  |  Morais, VA., et al. 2006. Biochim Biophys Acta. 1762: 802-10. PMID: 16938437
6. Deletion of 3 residues from the C-terminus of MCFD2 affects binding to ERGIC-53 and causes combined factor V and factor VIII deficiency.  |  Nyfeler, B., et al. 2008. Blood. 111: 1299-301. PMID: 17971482
7. Molecular basis of sugar recognition by the human L-type lectins ERGIC-53, VIPL, and VIP36.  |  Kamiya, Y., et al. 2008. J Biol Chem. 283: 1857-61. PMID: 18025080
8. Multistep, sequential control of the trafficking and function of the multiple sulfatase deficiency gene product, SUMF1 by PDI, ERGIC-53 and ERp44.  |  Fraldi, A., et al. 2008. Hum Mol Genet. 17: 2610-21. PMID: 18508857
9. Recycling of the endoplasmic reticulum/Golgi intermediate compartment protein ERGIC-53 in the secretory pathway.  |  Itin, C., et al. 1995. Biochem Soc Trans. 23: 541-4. PMID: 8566411
10. Mutations in the ER-Golgi intermediate compartment protein ERGIC-53 cause combined deficiency of coagulation factors V and VIII.  |  Nichols, WC., et al. 1998. Cell. 93: 61-70. PMID: 9546392

The preferred ERGIC-53 antibody is ERGIC-53 Antibody (C-6): sc-365158.