ERGIC-3 Antibody (B-5): sc-398778
- ERGIC-3 Antibody (B-5) is a mouse monoclonal IgG2b κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 171-194 within an internal region of ERGIC-3 of human origin
- recommended for detection of ERGIC-3 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ERGIC-3 Antibody (B-5) for WB applications. These reagents are now offered in bundles with ERGIC-3 Antibody (B-5) (see ordering information below).
ERGIC-3 Antibody (B-5) is a mouse monoclonal IgG2b antibody that detects ERGIC-3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ERGIC-3, also known as Erv46, is a critical component of the early secretory pathway, playing a vital role in the transport of newly synthesized glycoproteins from the endoplasmic reticulum to the Golgi apparatus. ERGIC-3 is primarily located in the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and is essential for maintaining the integrity of membrane trafficking processes. ERGIC-3 interacts with other proteins such as ERGIC-2 (Erv41) and ERGIC-1 (ERGIC32), forming a complex that facilitates selective cargo transport. Disruptions in this transport mechanism can lead to various cellular dysfunctions and diseases, highlighting ERGIC-3′s importance in cellular homeostasis and protein processing. Anti-ERGIC-3 antibody (B-5) provides valuable insights into the dynamics of the secretory pathway for researchers studying protein transport and related cellular processes.
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ERGIC-3 Antibody (B-5) References:
- The Erv41p-Erv46p complex: multiple export signals are required in trans for COPII-dependent transport from the ER. | Otte, S. and Barlowe, C. 2002. EMBO J. 21: 6095-104. PMID: 12426381
- Mammalian Erv46 localizes to the endoplasmic reticulum-Golgi intermediate compartment and to cis-Golgi cisternae. | Orci, L., et al. 2003. Proc Natl Acad Sci U S A. 100: 4586-91. PMID: 12663859
- Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46. | Breuza, L., et al. 2004. J Biol Chem. 279: 47242-53. PMID: 15308636
- Genetic and molecular interactions of the Erv41p-Erv46p complex involved in transport between the endoplasmic reticulum and Golgi complex. | Welsh, LM., et al. 2006. J Cell Sci. 119: 4730-40. PMID: 17077122
- Distinct adaptor proteins assist exit of Kre2-family proteins from the yeast ER. | Noda, Y., et al. 2014. Biol Open. 3: 209-24. PMID: 24585773
- The Erv41-Erv46 complex serves as a retrograde receptor to retrieve escaped ER proteins. | Shibuya, A., et al. 2015. J Cell Biol. 208: 197-209. PMID: 25583996
- The E3 ubiquitin ligase MARCH2 regulates ERGIC3-dependent trafficking of secretory proteins. | Yoo, W., et al. 2019. J Biol Chem. 294: 10900-10912. PMID: 31142615
- Molecular dissection of the Erv41-Erv46 retrograde receptor reveals a conserved cysteine-rich region in Erv46 required for retrieval activity. | Keiser, KJ. and Barlowe, C. 2020. Mol Biol Cell. 31: 209-220. PMID: 31825724
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ERGIC-3 Antibody (B-5) | sc-398778 | 200 µg/ml | $316.00 | |||
ERGIC-3 Antibody (B-5): m-IgGκ BP-HRP Bundle | sc-536149 | 200 µg Ab; 40 µg BP | $354.00 | |||
ERGIC-3 Antibody (B-5): m-IgG2b BP-HRP Bundle | sc-549518 | 200 µg Ab; 10 µg BP | $354.00 | |||
ERGIC-3 (B-5) Neutralizing Peptide | sc-398778 P | 100 µg/0.5 ml | $68.00 |