eRF1 Antibody (G-1): sc-390428
- eRF1 Antibody (G-1) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of eRF1 of human origin
- recommended for detection of eRF1 of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine, porcine and avian
- m-IgG2a BP-HRP is the preferred secondary detection reagent for eRF1 Antibody (G-1) for WB applications. This reagent is now offered in a bundle with eRF1 Antibody (G-1) (see ordering information below).
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eRF1 Antibody (G-1) is a mouse monoclonal IgG2a antibody that detects eRF1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). eRF1, or eukaryotic release factor 1, plays a crucial role in the termination of protein synthesis, a vital process for cellular function and homeostasis. eRF1 is located in the cytoplasm, where eRF1 interacts with the ribosome to facilitate the release of newly synthesized polypeptides upon encountering stop codons. Proper functioning of eRF1 is essential, as any disruption in this process can lead to the accumulation of incomplete proteins, potentially resulting in cellular stress or disease. eRF1 forms a quaternary complex with the GTP-binding protein eRF3, which enhances activity and ensures accurate termination of translation. The gene encoding human eRF1 is located on chromosome 5q31.2, and eRF1′s highly conserved structure, including the essential Asn-Ile-Lys-Ser (NIKS) tetrapeptide, underscores importance in maintaining the fidelity of protein synthesis across different species. With effective detection capabilities in various applications, anti-eRF1 antibody (G-1) serves as an invaluable tool for researchers studying the intricacies of translation termination and its implications in health and disease.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eRF1 Antibody (G-1) | sc-390428 | 200 µg/ml | $322.00 | |||
eRF1 Antibody (G-1): m-IgG2a BP-HRP Bundle | sc-546071 | 200 µg Ab; 10 µg BP | $361.00 |