Date published: 2026-10-17

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Estrogen Receptor beta 抗体 (B-3): sc-373853

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说明书
  • Estrogen Receptor beta 抗体 B-3 是小鼠单克隆 IgG2b κ,Estrogen Receptor beta抗体, 在28篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的ERβ的N-terminus的氨基酸2-29之间
  • Estrogen Receptor beta 抗体 (B-3) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 ERβ
  • 抗Estrogen Receptor beta抗体(B-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-373853 X, 200 µg/0.1 ml)
  • 2b BP-HRP">m-IgG2b BP-HRP是Estrogen Receptor beta Antibody (B-3) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与Estrogen Receptor beta Antibody (B-3) 打包提供(请参阅下面的订购信息)。
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関連項目

ERβ抗体(B-3)是一种IgG2b κ小鼠单克隆ERβ抗体(也称为雌激素受体β抗体、ERβ抗体、ESR2抗体或雌激素受体2抗体),可通过WB、IP、IF、IHC(P)和ELISA检测人源ERβ蛋白。ERβ抗体(B-3)既可作为非偶联的抗ERβ抗体形式,也可作为多种偶联形式的抗ERβ抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。雌激素受体(ER)是配体激活的转录因子类固醇/甲状腺激素受体超家族的成员。包括ERα和ERβ在内的雌激素受体含有DNA结合和配体结合结构域,在调节生殖组织的正常功能中起着至关重要的作用。它们位于细胞核中,但一些雌激素受体与细胞表面膜结合,并且可以通过细胞暴露于雌激素而迅速激活。已证明ERα和ERβ可被各种配体特异性激活。受体-配体相互作用会引发一系列事件,包括与热休克蛋白的解离、受体二聚化、磷酸化和激素激活受体与靶基因中特定调控元件的结合。有证据表明,尽管ERα和ERβ具有许多功能特征,但它们可能受到不同机制的调节。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Estrogen Receptor beta 抗体 (B-3) 参考文献:

  1. ERbeta信号在卵巢中的重要性。  |  Drummond, AE. and Fuller, PJ. 2010. J Endocrinol. 205: 15-23. PMID: 20019181
  2. Ahi1通过涉及抑郁行为性别差异的GR/ERβ/TPH2途径调节血清素分泌  |  Wang, B., et al. 2022. Cell Commun Signal. 20: 74. PMID: 35643536
  3. FoxA2通过转录抑制IGF2BP1来抑制子宫内膜异位症中ERβ介导的热解作用。  |  Feng, Y., et al. 2023. Exp Cell Res. 426: 113539. PMID: 36889571
  4. 雄性小鼠肝脏中17α-雌二醇的代谢益处部分由ERβ介导。  |  Mondal, SA., et al. 2023. Sci Rep. 13: 9841. PMID: 37330610
  5. WFDC3通过促进ERβ介导的TGFBR1转录抑制,抑制结肠直肠癌的肿瘤转移。  |  Liu, T., et al. 2023. Cell Death Dis. 14: 425. PMID: 37443102
  6. ERβ异构体在三阴性乳腺癌中的不同特征:进展和对进一步研究的影响。  |  Yan, S., et al. 2023. Front Cell Dev Biol. 11: 1240386. PMID: 37936981
  7. 卵巢ERβ染色质和转录组揭示了与LRH-1的染色质相互作用。  |  Birgersson, M., et al. 2023. BMC Biol. 21: 277. PMID: 38031019
  8. 苯并[a]芘暴露通过调节ERβ介导的LINC02977转录影响结直肠癌的易感性。  |  Ben, S., et al. 2024. Environ Int. 184: 108443. PMID: 38277997
  9. AURKA 通过ERβ促进卵巢子宫内膜异位症的糖酵解和发育。  |  Sun, Y., et al. 2024. Endocrinology. 165: PMID: 38340326
  10. 二甘醇丙腈刺激的ERβ核积累通过与FOXO3A相互作用促进MyoD诱导的mdx小鼠肌肉再生。  |  Tong, H., et al. 2024. Pharmacol Res. 208: 107376. PMID: 39216837

订购信息

产品名称产品编号规格价格数量收藏夹

Estrogen Receptor beta 抗体 (B-3)

sc-373853
200 µg/ml
$322.00

Estrogen Receptor beta (B-3): m-IgG2b BP-HRP 套装

sc-548734
200 µg Ab; 10 µg BP
$361.00

Estrogen Receptor beta 抗体 (B-3) X

sc-373853 X
200 µg/0.1 ml
$322.00

Estrogen Receptor beta 抗体 (B-3) AC

sc-373853 AC
500 µg/ml, 25% agarose
$424.00

Estrogen Receptor beta 抗体 (B-3) HRP

sc-373853 HRP
200 µg/ml
$322.00

Estrogen Receptor beta 抗体 (B-3) FITC

sc-373853 FITC
200 µg/ml
$336.00

Estrogen Receptor beta 抗体 (B-3) PE

sc-373853 PE
200 µg/ml
$349.00

Estrogen Receptor beta 抗体 (B-3) Alexa Fluor® 488

sc-373853 AF488
200 µg/ml
$364.00

Estrogen Receptor beta 抗体 (B-3) Alexa Fluor® 546

sc-373853 AF546
200 µg/ml
$364.00

Estrogen Receptor beta 抗体 (B-3) Alexa Fluor® 594

sc-373853 AF594
200 µg/ml
$364.00

Estrogen Receptor beta 抗体 (B-3) Alexa Fluor® 647

sc-373853 AF647
200 µg/ml
$364.00

Estrogen Receptor beta 抗体 (B-3) Alexa Fluor® 680

sc-373853 AF680
200 µg/ml
$364.00

Estrogen Receptor beta 抗体 (B-3) Alexa Fluor® 790

sc-373853 AF790
200 µg/ml
$364.00

Estrogen Receptor beta (B-3) 中和勝肽

sc-373853 P
100 µg/0.5 ml
$69.00

is this suitable for mouse tissues IF staining?

Asked by: Alexliang
Thank you for your question. We have not yet tested this antibody, ERβ (B-3): sc-373853, on mouse tissues so it is currently not recommended for use in that species. As an alternative, we would recommend a different antibody, ERβ (B-1): sc-390243. https://www.scbt.com/scbt/product/erbeta-antibody-b-1
Answered by: Technical Support
Date published: 2017-11-15

Can ERb(B-3): sc-373853 monoclonal antibody be used to stain free floating sections? Where could I find the full protocol? Thank you.

Asked by: Bren
Thank you for your question. This mouse monoclonal antibody, ERβ (B-3): sc-373853, is suitable for IF and IHC(P). We have not tested the antibody specifically on free floating sections, so we do not have a protocol for this specific application. However, based on the antibody's performance in the other applications, we do believe it should be suitable. A link to our general protocol for using this antibody is provided below, and may be used as a reference. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-08-18

Can ERβ (B-3): sc-373853 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, ERβ (B-3): sc-373853 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 3
Date published: 2017-01-10
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Rated 2 out of 5 by 12345abcd from Not really goodNot really good for melanoma cell lines, I see result at different kda (90kDa).
Date published: 2021-07-07
Rated 4 out of 5 by agnes2007 from Great productI got a free sample of this antibody. It works good.
Date published: 2019-08-13
Rated 5 out of 5 by Kayb from Worked Good for Western BlotWorked good for western blot with 1:200 dilution (75 ug protein, rat liver, using the recommended Santa Cruz secondary).
Date published: 2019-07-26
Rated 3 out of 5 by scientist4 from Looks ok but not sure if that just one band or twoTested this antibody for Western Blot with human cells since there are very few ERb validated antibodies available. Got a band that is the right size (around 55 kDa) (depicted as top band in figure) but it is not very crisp. I wonder if that is two bands mushed together. But overall happy with the results compared to other antibodies. (Bottom band in figure is bActin)
Date published: 2018-03-20
Rated 5 out of 5 by LVH15 from Worked great for cell and tissue lysates for WB!Tested on human aortic SMC lysates and aortic tissue lysates at 1:100 dilution for WB.
Date published: 2018-02-28
Rated 1 out of 5 by かおりっくす from Ishikawa細胞ではいまひとつです。Ishikawa細胞のタンパクを10μg applyしました。あまりきれいにbandが出ませんでした。
Date published: 2018-02-23
Rated 3 out of 5 by Jenny8823 from produced immunofluorescence in rat brain sectionsantibody: 1:50 dilution Brains were fixed by perfusion with 4% paraformaldehyde It produced immunofluorecence.
Date published: 2017-08-30
Rated 5 out of 5 by wgn20041986 from Good antibodyI have ordered a free sample, and I got clear band of MCF-7 xenograft. I developed 5 mins because a low expression.
Date published: 2017-08-23
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Estrogen Receptor beta 抗体 (B-3) is rated 3.5 out of 5 by 18.
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