ER beta Antibody (B-3) recommended for detection of ERβ of human origin by WB, IP, IF, IHC(P) and ELISA

ERβ Antibody (B-3): sc-373853

ERβ Antibody (B-3) is rated 3.6 out of 5 by 17.
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Datasheets

    • Anti-ERβ Antibody (B-3) is a mouse monoclonal IgG2b (kappa light chain) ERβ antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 2-29 at the N-terminus of ERβ of human origin
    • ER beta Antibody (B-3) is recommended for detection of ERβ of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-ER beta Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-373853 X, 200 µg/0.1 ml)
    • blocking peptide, sc-373853 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ERβ (B-3): sc-373853.
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is this suitable for mouse tissues IF staining?

Asked by: Alexliang
Thank you for your question. We have not yet tested this antibody, ERβ (B-3): sc-373853, on mouse tissues so it is currently not recommended for use in that species. As an alternative, we would recommend a different antibody, ERβ (B-1): sc-390243. https://www.scbt.com/scbt/product/erbeta-antibody-b-1
Answered by: Technical Support
Date published: 2017-11-15

Can ERb(B-3): sc-373853 monoclonal antibody be used to stain free floating sections? Where could I find the full protocol? Thank you.

Asked by: Bren
Thank you for your question. This mouse monoclonal antibody, ERβ (B-3): sc-373853, is suitable for IF and IHC(P). We have not tested the antibody specifically on free floating sections, so we do not have a protocol for this specific application. However, based on the antibody's performance in the other applications, we do believe it should be suitable. A link to our general protocol for using this antibody is provided below, and may be used as a reference. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-08-18

Can ERβ (B-3): sc-373853 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, ERβ (B-3): sc-373853 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 3
Date published: 2017-01-10
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Rated 4 out of 5 by from Great product I got a free sample of this antibody. It works good.
Date published: 2019-08-13
Rated 5 out of 5 by from Worked Good for Western Blot Worked good for western blot with 1:200 dilution (75 ug protein, rat liver, using the recommended Santa Cruz secondary).
Date published: 2019-07-26
Rated 3 out of 5 by from Looks ok but not sure if that just one band or two Tested this antibody for Western Blot with human cells since there are very few ERb validated antibodies available. Got a band that is the right size (around 55 kDa) (depicted as top band in figure) but it is not very crisp. I wonder if that is two bands mushed together. But overall happy with the results compared to other antibodies. (Bottom band in figure is bActin)
Date published: 2018-03-20
Rated 5 out of 5 by from Worked great for cell and tissue lysates for WB! Tested on human aortic SMC lysates and aortic tissue lysates at 1:100 dilution for WB.
Date published: 2018-02-28
Rated 1 out of 5 by from Ishikawa細胞ではいまひとつです。 Ishikawa細胞のタンパクを10μg applyしました。あまりきれいにbandが出ませんでした。
Date published: 2018-02-23
Rated 3 out of 5 by from produced immunofluorescence in rat brain sections antibody: 1:50 dilution Brains were fixed by perfusion with 4% paraformaldehyde It produced immunofluorecence.
Date published: 2017-08-30
Rated 5 out of 5 by from Good antibody I have ordered a free sample, and I got clear band of MCF-7 xenograft. I developed 5 mins because a low expression.
Date published: 2017-08-23
Rated 3 out of 5 by from Santa 抗体不错,性价比高! 我试过这只抗体,稀释比例是1:100, 检测了ER+的乳腺癌细胞,条带清楚,可用。Santa 抗量多,实惠。
Date published: 2017-07-11
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