ErbB3/HER3 CRISPR/Cas9 KO Plasmid (m): sc-420219
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ErbB3/HER3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ErbB3/HER3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ErbB3/HER3 Antibody (G-4): sc-7390
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ErbB3/HER3 CRISPR/Cas9 KO Plasmid (m) | sc-420219 | 20 µg | $397.00 |
Overview
Erbb3 encodes the receptor tyrosine kinase ErbB3/HER3, a member of the EGFR/ErbB family that preferentially forms heterodimers with ERBB2 to transduce growth factor signals. Despite limited intrinsic kinase activity, ErbB3 provides multiple docking sites for PI3K and strongly couples ligand stimulation to PI3K–AKT signaling, with additional crosstalk into MAPK/ERK and mTOR-regulated processes. In mouse cells, ErbB3 contributes to developmental and tissue homeostasis programs, influencing epithelial differentiation, survival, and migration through receptor trafficking and feedback regulation. Dysregulated ERBB signaling involving HER3 is frequently studied in contexts of oncogenic receptor network remodeling and resistance-like signaling adaptations, making Erbb3 a common node for pathway interrogation.
ErbB3/HER3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Erbb3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Erbb3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Erbb3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ErbB3/HER3 protein expression.
This CRISPR knockout system enables efficient generation of Erbb3-deficient cell models for investigation of ErbB3/HER3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Erbb3 exon(s) critical for ErbB3/HER3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Erbb3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ErbB3/HER3 CRISPR/Cas9 KO Plasmid (m) and ErbB3/HER3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Erbb3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ErbB3/HER3 HDR Plasmid (m) and ErbB3/HER3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Erbb3 homology arms to support homology-directed repair at defined Erbb3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.