ErbB3/HER3 Antibody (RTJ.2): sc-415

ErbB-3 Antibody (RTJ.2) is a mouse monoclonal IgG2b kappa light chain antibody raised against the cytoplasmic domain of human ErbB-3, a crucial member of the ErbB receptor tyrosine kinase family. ErbB-3 mouse monoclonal antibody (RTJ.2) recognizes an epitope within the cytoplasmic region and demonstrates broad species cross-reactivity, detecting ErbB-3 in mouse, rat, bovine, porcine, and human samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry on paraffin-embedded sections (IHCP), and flow cytometry (FCM) applications. Anti-ErbB-3 monoclonal antibody (RTJ.2) is available in non-conjugated form and multiple conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, offering versatility for diverse experimental needs. ErbB-3, also known as FERLK, HER3, LCCS2, MDA-BF-1, VSCN1, erbB3-S, and ERBB3, maintains precise localization at the plasma membrane, where ErbB-3 orchestrates sophisticated cellular signaling networks. This strategic positioning enables ErbB-3 to respond to ligands like heregulin and form either homodimers or heterodimers with other family members, particularly ErbB-2 (HER2). These dimerization events trigger crucial downstream signaling cascades, notably the phosphoinositide 3-kinase (PI3K) pathway, which regulates cell growth, survival, and differentiation. The clinical significance of ErbB-3 becomes particularly evident in cancer biology, where ErbB-3 overexpression frequently occurs in various carcinomas, including human mammary tumors. This dysregulation enhances oncogenic processes and contributes to therapeutic resistance. Through these diverse cellular functions, ErbB-3 (RTJ.2) antibody provides researchers with an invaluable tool for investigating cancer development mechanisms and advancing targeted therapeutic strategies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ErbB3/HER3 Antibody (RTJ.2) has 61 citations in a variety of scientific publications.

Primary antibodies like Anti-ErbB3/HER3 Antibody (RTJ.2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ErbB3/HER3 Antibody (RTJ.2) Specifications

Alternate Product Name: HER3

Product Clone Name: RTJ.2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: ErbB-3

Epitope Species: human

Product Reactivity/Detection: ErbB-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 61 citations in various scientific publications

Available Conjugates/Forms for ErbB3/HER3 Antibody (RTJ.2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin

The preferred ErbB3/HER3 antibody is ErbB3/HER3 Antibody (G-4): sc-7390.