ERAP1 Antibody (B-10): sc-271823

ERAP1 Antibody (B-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects ERAP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-ERAP1 antibody (B-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The endoplasmic reticulum aminopeptidase 1 (ERAP1) is crucially located in the lumen of the endoplasmic reticulum, where ERAP1 plays a vital role in processing antigenic precursors for major histocompatibility complex (MHC) class I peptide presentation. Specifically, ERAP1 trims peptides to the optimal length of eight to eleven amino acids, which is essential for effective immune recognition and response. ERAP1 is induced by interferon-g and is encoded by the ARTS1 gene located on human chromosome 5q15. In addition to antigen processing, ERAP1 is involved in the inactivation of several bioactive peptides, such as angiotensin II, which may influence blood pressure regulation. Furthermore, ERAP1 has been implicated in angiogenesis, as ERAP1 regulates the proliferation and migration of endothelial cells, and is characterized as a tumor necrosis factor receptor 1 (TNFR1) binding protein that promotes TNFR1 shedding, highlighting ERAP1′s multifaceted role in both immune response and vascular biology.

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ERAP1 Antibody (B-10) References:

1. Characterization of recombinant human adipocyte-derived leucine aminopeptidase expressed in Chinese hamster ovary cells.  |  Hattori, A., et al. 2000. J Biochem. 128: 755-62. PMID: 11056387
2. Genomic organization of the human adipocyte-derived leucine aminopeptidase gene and its relationship to the placental leucine aminopeptidase/oxytocinase gene.  |  Hattori, A., et al. 2001. J Biochem. 130: 235-41. PMID: 11481040
3. Identification of 33 polymorphisms in the adipocyte-derived leucine aminopeptidase (ALAP) gene and possible association with hypertension.  |  Yamamoto, N., et al. 2002. Hum Mutat. 19: 251-7. PMID: 11857741
4. Identification of ARTS-1 as a novel TNFR1-binding protein that promotes TNFR1 ectodomain shedding.  |  Cui, X., et al. 2002. J Clin Invest. 110: 515-26. PMID: 12189246
5. Puromycin insensitive leucyl-specific aminopeptidase (PILSAP) is involved in the activation of endothelial integrins.  |  Akada, T., et al. 2002. J Cell Physiol. 193: 253-62. PMID: 12385003
6. An IFN-gamma-induced aminopeptidase in the ER, ERAP1, trims precursors to MHC class I-presented peptides.  |  Saric, T., et al. 2002. Nat Immunol. 3: 1169-76. PMID: 12436109
7. The ER aminopeptidase ERAP1 enhances or limits antigen presentation by trimming epitopes to 8-9 residues.  |  York, IA., et al. 2002. Nat Immunol. 3: 1177-84. PMID: 12436110
8. ERAP1-ERAP2 dimerization increases peptide-trimming efficiency.  |  Evnouchidou, I., et al. 2014. J Immunol. 193: 901-8. PMID: 24928998
9. How ERAP1 and ERAP2 Shape the Peptidomes of Disease-Associated MHC-I Proteins.  |  López de Castro, JA. 2018. Front Immunol. 9: 2463. PMID: 30425713
10. Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity Via Inhibiting USP33-Mediated ADRB2 Deubiquitination.  |  Niu, Y., et al. 2022. Diabetes. 71: 921-933. PMID: 35192681
11. The emerging multifunctional roles of ERAP1, ERAP2 and IRAP between antigen processing and renin-angiotensin system modulation.  |  Mattorre, B., et al. 2022. Front Immunol. 13: 1002375. PMID: 36203608
12. Shedding new light on the role of ERAP1 in Type 1 diabetes: A perspective on disease management.  |  Paldino, G. and Fierabracci, A. 2023. Autoimmun Rev. 22: 103291. PMID: 36740089

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ERAP1 Antibody (B-10) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-ERAP1 Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ERAP1 Antibody (B-10) Specifications

Alternate Product Name: ERAP1

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 721-840

Epitope Target: ERAP1

Epitope Species: human

Product Reactivity/Detection: ERAP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for ERAP1 Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin