Date published: 2026-4-28

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eqbJ Antibody (A-5): sc-377247

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Datasheets
  • eqbJ Antibody (A-5) is a mouse monoclonal IgG1 (lambda light chain) provided at 200 µg/ml
  • raised against amino acids 1-225 of Streptococcus equi subsp. equi origin
  • recommended for detection of ABC transporter, ATP-binding component (eqbJ) of Streptococcus equi subsp. equi 4047 origin of S. equi origin by WB, IP, IF and ELISA
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for eqbJ Antibody (A-5) for WB applications. These reagents are now offered in bundles with eqbJ Antibody (A-5) (see ordering information below).
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    eqbJ Antibody (A-5) is a mouse monoclonal IgG1 antibody that detects EqbJ protein of Streptococcus equi origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-EqbJ antibody (A-5) targets a 458 amino acid protein that plays a crucial role in the pathogenesis of Streptococcus equi, a clonal pathogen specifically adapted to equine hosts, which causes the highly contagious disease known as strangles. EqbJ (A-5) antibody recognizes a protein involved in the transport of essential molecules across the bacterial membrane, facilitating nutrient uptake and contributing to bacterial survival and virulence. Understanding EqbJ function is vital for developing effective vaccines and diagnostic tools while providing insights into bacterial pathogenesis mechanisms. EqbJ presence on S. equi surface makes this protein a potential biomarker for serodiagnosis, enabling identification of infected animals and implementation of control measures to prevent strangles spread. Through interactions with host proteins, EqbJ may also contribute to immune response evasion, highlighting its significance in equine infectious disease research.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    eqbJ Antibody (A-5)

    sc-377247
    200 µg/ml
    $322.00

    eqbJ Antibody (A-5): m-IgG Fc BP-HRP Bundle

    sc-540707
    200 µg Ab; 10 µg BP
    $361.00

    eqbJ Antibody (A-5): m-IgG1 BP-HRP Bundle

    sc-542313
    200 µg Ab; 20 µg BP
    $361.00

    How much sample do you recommend loading on the gel for Western blot detection using eqbJ (A-5): sc-377247?

    Asked by: AbPolly
    We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by from Produced positive Western Blot data of eqbJProduced positive Western Blot data of eqbJ expression in Streptococcus equi whole cell lysate. -SCBT QC
    Date published: 2014-04-05
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    eqbJ Antibody (A-5) is rated 5.0 out of 5 by 1.
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