epsin 1 Antibody (C-11): sc-55556

Epsin 1 Antibody (C-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects epsin 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-epsin 1 antibody (C-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Epsin 1 (EPN1) plays a crucial role in the endocytic process, particularly in forming Clathrin-coated vesicles, which are essential for membrane proteins and lipids internalization. EPN1 is primarily located at the leading edge of vesicular coated pits, where EPN1 facilitates membrane bending, a critical step in endocytosis. This localization ensures efficient uptake of extracellular materials and signaling molecules, thereby influencing cellular communication and nutrient absorption. EPN1 also interacts with key proteins such as Eps15 and Clathrin, and EPN1 function is regulated by post-translational modifications, including phosphorylation, which can modulate activity during different cellular states, such as mitosis. Understanding EPN1 dynamics and interactions is essential for elucidating endocytosis mechanisms and implications in various physiological and pathological processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

epsin 1 Antibody (C-11) References:

1. Epsin binds to the EH domain of POB1 and regulates receptor-mediated endocytosis.  |  Morinaka, K., et al. 1999. Oncogene. 18: 5915-22. PMID: 10557078
2. The epsins define a family of proteins that interact with components of the clathrin coat and contain a new protein module.  |  Rosenthal, JA., et al. 1999. J Biol Chem. 274: 33959-65. PMID: 10567358
3. Epsin binds to clathrin by associating directly with the clathrin-terminal domain. Evidence for cooperative binding through two discrete sites.  |  Drake, MT., et al. 2000. J Biol Chem. 275: 6479-89. PMID: 10692452
4. The ubiquitin-interacting motifs target the endocytic adaptor protein epsin for ubiquitination.  |  Oldham, CE., et al. 2002. Curr Biol. 12: 1112-6. PMID: 12121618
5. Curvature of clathrin-coated pits driven by epsin.  |  Ford, MG., et al. 2002. Nature. 419: 361-6. PMID: 12353027
6. Epsins: adaptors in endocytosis?  |  Wendland, B. 2002. Nat Rev Mol Cell Biol. 3: 971-7. PMID: 12461563
7. Contrasting membrane interaction mechanisms of AP180 N-terminal homology (ANTH) and epsin N-terminal homology (ENTH) domains.  |  Stahelin, RV., et al. 2003. J Biol Chem. 278: 28993-9. PMID: 12740367
8. A role for epsin N-terminal homology/AP180 N-terminal homology (ENTH/ANTH) domains in tubulin binding.  |  Hussain, NK., et al. 2003. J Biol Chem. 278: 28823-30. PMID: 12750376
9. Tetanus toxin is internalized by a sequential clathrin-dependent mechanism initiated within lipid microdomains and independent of epsin1.  |  Deinhardt, K., et al. 2006. J Cell Biol. 174: 459-71. PMID: 16880274
10. EPSIN1 Modulates the Plasma Membrane Abundance of FLAGELLIN SENSING2 for Effective Immune Responses.  |  Collins, CA., et al. 2020. Plant Physiol. 182: 1762-1775. PMID: 32094305
11. Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis.  |  Chen, H., et al. 1998. Nature. 394: 793-7. PMID: 9723620

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-epsin 1 Antibody (C-11) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-epsin 1 Antibody (C-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

epsin 1 Antibody (C-11) Specifications

Alternate Product Name: epsin 1

Product Clone Name: C-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 311-440

Epitope Target: epsin 1

Epitope Species: human

Product Reactivity/Detection: epsin 1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for epsin 1 Antibody (C-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin