epsin 1 Antibody (A-9): sc-166354
- epsin 1 Antibody (A-9) is a mouse monoclonal IgG3 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 523-552 near the C-terminus of epsin 1 of rat origin
- recommended for detection of epsin 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG3 BP-HRP is the preferred secondary detection reagent for epsin 1 Antibody (A-9) for WB and IHC(P) applications. This reagent is now offered in a bundle with epsin 1 Antibody (A-9) (see ordering information below).
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Epsin 1 Antibody (A-9) is a mouse monoclonal IgG3 antibody that detects Epsin 1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Epsin 1 plays a crucial role in the endocytic process, particularly in the formation of Clathrin-coated vesicles, which are essential for the internalization of membrane proteins and lipids. Epsin 1 is primarily located at the leading edge of vesicular coated pits, where Epsin 1 facilitates the bending of the membrane, a critical step in the endocytosis pathway. The presence of an epsin N-terminal homology (ENTH) domain and a Clathrin-binding motif within Epsin 1 underscores its importance in the recruitment of Clathrin and other accessory proteins, thereby regulating the dynamics of vesicle formation. Additionally, Epsin 1 undergoes post-translational modifications, including phosphorylation, which can influence interaction with other proteins and functional activity during cellular processes. Anti-Epsin 1 antibody (A-9) enables researchers to gain valuable insights into the mechanisms of endocytosis and the role of Epsin 1 in various physiological and pathological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
epsin 1 Antibody (A-9) References:
- Nucleocytoplasmic shuttling of endocytic proteins. | Vecchi, M., et al. 2001. J Cell Biol. 153: 1511-7. PMID: 11425879
- Flotillin-dependent clustering of the amyloid precursor protein regulates its endocytosis and amyloidogenic processing in neurons. | Schneider, A., et al. 2008. J Neurosci. 28: 2874-82. PMID: 18337418
- γ-secretase-dependent cleavage initiates notch signaling from the plasma membrane. | Sorensen, EB. and Conner, SD. 2010. Traffic. 11: 1234-45. PMID: 20573067
- Neuronal activity and the expression of clathrin-assembly protein AP180. | Wu, F., et al. 2010. Biochem Biophys Res Commun. 402: 297-300. PMID: 20937255
- In situ quantitative imaging of cellular lipids using molecular sensors. | Yoon, Y., et al. 2011. Nat Chem. 3: 868-74. PMID: 22024883
- Preubiquitinated chimeric ErbB2 is constitutively endocytosed and subsequently degraded in lysosomes. | Vuong, TT., et al. 2013. Exp Cell Res. 319: 32-45. PMID: 23127513
- Epsin 1 Promotes Synaptic Growth by Enhancing BMP Signal Levels in Motoneuron Nuclei. | Vanlandingham, PA., et al. 2013. PLoS One. 8: e65997. PMID: 23840387
- Cooperativity of membrane-protein and protein-protein interactions control membrane remodeling by epsin 1 and affects clathrin-mediated endocytosis. | Kroppen, B., et al. 2021. Cell Mol Life Sci. 78: 2355-2370. PMID: 32997199
- Structural Dissection of Epsin-1 N-Terminal Helical Peptide: The Role of Hydrophobic Residues in Modulating Membrane Curvature. | Nishimura, M., et al. 2023. Chemistry. 29: e202300129. PMID: 36878866
- A single-particle analysis method for detecting membrane remodelling and curvature sensing. | Colussi, A., et al. 2024. J Cell Sci. 137: PMID: 39324332
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
epsin 1 Antibody (A-9) | sc-166354 | 200 µg/ml | $322.00 | |||
epsin 1 Antibody (A-9): m-IgG3 BP-HRP Bundle | sc-551980 | 200 µg Ab; 40 µg BP | $361.00 | |||
epsin 1 (A-9) Neutralizing Peptide | sc-166354 P | 100 µg/0.5 ml | $69.00 |