Eps8 Antibody (F-8): sc-390257

Eps8 Antibody (F-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects Eps8 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Eps8 antibody (F-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Eps8 plays a crucial role in cellular signaling, particularly in the context of receptor tyrosine kinases (RTKs), which are essential for regulating cell growth and proliferation. Eps8 is primarily located in the cytoplasm and at the cell membrane, where Eps8 interacts with various signaling molecules, thereby influencing actin dynamics and cellular morphology. This localization is vital as Eps8 participates in the modulation of cytoskeletal rearrangements in response to extracellular signals, which is critical for processes such as cell migration and division. Dysregulation of Eps8 and associated pathways can lead to aberrant cell behavior and contribute to oncogenesis, highlighting the importance of studying Eps8 in cancer research. Furthermore, Eps8 has been shown to associate with the epidermal growth factor receptor, despite lacking a functional SH2 domain, and contains both a pleckstrin homology domain and a SH3 domain, the roles of which are still being investigated.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Eps8 Antibody (F-8) References:

1. Differential expression of epidermal growth factor-related proteins in human colorectal tumors.  |  Ciardiello, F., et al. 1991. Proc Natl Acad Sci U S A. 88: 7792-6. PMID: 1715580
2. Human transforming growth factors induce tyrosine phosphorylation of EGF receptors.  |  Reynolds, FH., et al. 1981. Nature. 292: 259-62. PMID: 6265789
3. Direct binding of eps8 to the juxtamembrane domain of EGFR is phosphotyrosine- and SH2-independent.  |  Castagnino, P., et al. 1995. Oncogene. 10: 723-9. PMID: 7532293
4. eps15, a novel tyrosine kinase substrate, exhibits transforming activity.  |  Fazioli, F., et al. 1993. Mol Cell Biol. 13: 5814-28. PMID: 7689153
5. The SH3 domain of Crk binds specifically to a conserved proline-rich motif in Eps15 and Eps15R.  |  Schumacher, C., et al. 1995. J Biol Chem. 270: 15341-7. PMID: 7797522
6. Evolutionary conservation of the EPS8 gene and its mapping to human chromosome 12q23-q24.  |  Wong, WT., et al. 1994. Oncogene. 9: 3057-61. PMID: 8084614
7. Eps8, a substrate for the epidermal growth factor receptor kinase, enhances EGF-dependent mitogenic signals.  |  Fazioli, F., et al. 1993. EMBO J. 12: 3799-808. PMID: 8404850

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Eps8 Antibody (F-8) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Eps8 Antibody (F-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Eps8 Antibody (F-8) Specifications

Alternate Product Name: Eps8

Product Clone Name: F-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 583-821

Epitope Target: Eps8

Epitope Species: mouse

Product Reactivity/Detection: Eps8

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Eps8 Antibody (F-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin