Eps15 Antibody (E-3): sc-390259

Eps15 Antibody (E-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects Eps15 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Eps15 antibody (E-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Eps15 plays a crucial role in regulating cellular processes, particularly in receptor tyrosine kinase (RTK) signaling pathways, which are essential for cell growth and differentiation. Eps15 interacts with the amino-terminal portion of Crk through a conserved proline-rich domain, a characteristic feature of Crk binding proteins. This interaction suggests Eps15 may be involved in downstream signaling events triggered by RTKs, which can influence cellular transformation and proliferation. Eps15 becomes tyrosine phosphorylated following EGF stimulation, and overexpression in NIH/3T3 cells has been linked to cellular transformation, highlighting potential role in oncogenic processes. Understanding Eps15 dynamics and interactions is vital for elucidating mechanisms underlying growth regulation and implications of dysregulated signaling in cancer development.

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Eps15 Antibody (E-3) References:

1. Differential expression of epidermal growth factor-related proteins in human colorectal tumors.  |  Ciardiello, F., et al. 1991. Proc Natl Acad Sci U S A. 88: 7792-6. PMID: 1715580
2. Eps15 mediates vesicle trafficking from the trans-Golgi network via an interaction with the clathrin adaptor AP-1.  |  Chi, S., et al. 2008. Mol Biol Cell. 19: 3564-75. PMID: 18524853
3. UIM domain-dependent recruitment of the endocytic adaptor protein Eps15 to ubiquitin-enriched endosomes.  |  Gucwa, AL. and Brown, DA. 2014. BMC Cell Biol. 15: 34. PMID: 25260758
4. Interaction of phosphorylated Rab11-FIP2 with Eps15 regulates apical junction composition.  |  Lapierre, LA., et al. 2017. Mol Biol Cell. 28: 1088-1100. PMID: 28228550
5. Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex.  |  Gu, L., et al. 2023. Pharmacol Res. 192: 106789. PMID: 37149115
6. Human transforming growth factors induce tyrosine phosphorylation of EGF receptors.  |  Reynolds, FH., et al. 1981. Nature. 292: 259-62. PMID: 6265789
7. Direct binding of eps8 to the juxtamembrane domain of EGFR is phosphotyrosine- and SH2-independent.  |  Castagnino, P., et al. 1995. Oncogene. 10: 723-9. PMID: 7532293
8. eps15, a novel tyrosine kinase substrate, exhibits transforming activity.  |  Fazioli, F., et al. 1993. Mol Cell Biol. 13: 5814-28. PMID: 7689153
9. The SH3 domain of Crk binds specifically to a conserved proline-rich motif in Eps15 and Eps15R.  |  Schumacher, C., et al. 1995. J Biol Chem. 270: 15341-7. PMID: 7797522
10. Evolutionary conservation of the EPS8 gene and its mapping to human chromosome 12q23-q24.  |  Wong, WT., et al. 1994. Oncogene. 9: 3057-61. PMID: 8084614
11. Eps8, a substrate for the epidermal growth factor receptor kinase, enhances EGF-dependent mitogenic signals.  |  Fazioli, F., et al. 1993. EMBO J. 12: 3799-808. PMID: 8404850
12. Association and colocalization of Eps15 with adaptor protein-2 and clathrin.  |  van Delft, S., et al. 1997. J Cell Biol. 136: 811-21. PMID: 9049247

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Eps15 Antibody (E-3) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Eps15 Antibody (E-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Eps15 Antibody (E-3) Specifications

Alternate Product Name: Eps15

Product Clone Name: E-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 791-825

Epitope Target: Eps15

Epitope Species: human

Product Reactivity/Detection: Eps15

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Eps15 Antibody (E-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin