EPLIN Antibody (20): sc-136399

EPLIN Antibody (20) is a mouse monoclonal IgG1 kappa light chain antibody that detects EPLIN protein of mouse, rat, human, and canine origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). EPLIN Antibody (20) is available in both non-conjugated and various conjugated forms, including agarose and horseradish peroxidase (HRP). Epithelial protein lost in neoplasm (EPLIN) plays a crucial role in regulating the actin cytoskeleton, which is essential for maintaining cell shape, motility, and integrity. EPLIN is characterized by a single centrally located lin-11, isl-1, and mec-3 (LIM) domain, along with at least two actin-binding domains, where the C-terminal domain exhibits stronger binding affinity than the N-terminal domain. By interacting with actin monomers and filaments, EPLIN enhances formation and stability of actin stress fibers, delays filament nucleation, and reduces formation of branched filaments, thereby promoting bundling of actin filaments. EPLIN inhibits membrane ruffling and prevents actin filament depolymerization, which is vital for cellular processes such as migration and adhesion. EPLIN expression is notably high in tissues such as placenta, kidney, pancreas, prostate, ovary, spleen, and heart, while lower levels are found in lung, liver, brain, skeletal muscle, thymus, testis, and intestine. EPLIN exists in two isoforms, EPLIN-alpha and EPLIN-beta, with downregulation of EPLIN-alpha being linked to increased motility of invasive tumor cells, highlighting its potential role in cancer progression.

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EPLIN Antibody (20) References:

1. EPLIN, epithelial protein lost in neoplasm.  |  Maul, RS. and Chang, DD. 1999. Oncogene. 18: 7838-41. PMID: 10618726
2. Characterization of the human EPLIN (Epithelial Protein Lost in Neoplasm) gene reveals distinct promoters for the two EPLIN isoforms.  |  Chen, S., et al. 2000. Gene. 248: 69-76. PMID: 10806352
3. Characterization of mouse epithelial protein lost in neoplasm (EPLIN) and comparison of mammalian and zebrafish EPLIN.  |  Maul, RS., et al. 2001. Gene. 262: 155-60. PMID: 11179679
4. Inhibition of anchorage-independent growth of transformed NIH3T3 cells by epithelial protein lost in neoplasm (EPLIN) requires localization of EPLIN to actin cytoskeleton.  |  Song, Y., et al. 2002. Mol Biol Cell. 13: 1408-16. PMID: 11950948
5. EPLIN regulates actin dynamics by cross-linking and stabilizing filaments.  |  Maul, RS., et al. 2003. J Cell Biol. 160: 399-407. PMID: 12566430
6. Compositional characterization of the cytoskeleton of NK-like cells.  |  Meng, X. and Wilkins, JA. 2005. J Proteome Res. 4: 2081-7. PMID: 16335953
7. EPLIN is a crucial regulator for extrusion of RasV12-transformed cells.  |  Ohoka, A., et al. 2015. J Cell Sci. 128: 781-9. PMID: 25609711
8. EPLIN Expression in Gastric Cancer and Impact on Prognosis and Chemoresistance.  |  Gong, W., et al. 2021. Biomolecules. 11: PMID: 33917939
9. Epithelial Protein Lost in Neoplasm, EPLIN, the Cellular and Molecular Prospects in Cancers.  |  Zeng, J., et al. 2021. Biomolecules. 11: PMID: 34356662
10. EPLIN, a Putative Tumour Suppressor in Colorectal Cancer, Implications in Drug Resistance.  |  Zeng, J., et al. 2022. Int J Mol Sci. 23: PMID: 36499558
11. EPLIN-β is a novel substrate of ornithine decarboxylase antizyme 1 and mediates cellular migration.  |  Li, D., et al. 2023. J Cell Sci. 136: PMID: 37325974
12. The concerted action of SEPT9 and EPLIN modulates the adhesion and migration of human fibroblasts.  |  Hecht, M., et al. 2024. Life Sci Alliance. 7: PMID: 38719752

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EPLIN Antibody (20) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-EPLIN Antibody (20) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EPLIN Antibody (20) Specifications

Alternate Product Name: EPLIN

Product Clone Name: 20

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-141

Epitope Target: EPLIN

Epitope Species: human

Product Reactivity/Detection: EPLIN

Species Reactivity/Detection: mouse, rat, human, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for EPLIN Antibody (20): AC: Agarose, HRP: Horseradish Peroxidase