EPI64 Antibody (B-9): sc-376991
- EPI64 Antibody (B-9) is a mouse monoclonal IgG1 κ EPI64 antibody provided at 200 µg/ml
- raised against amino acids 381-500 mapping at the C-terminus of EPI64 of mouse origin
- EPI64 Antibody (B-9) is recommended for detection of EPI64 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-EPI64 Antibody (B-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for EPI64 Antibody (B-9) for WB applications. These reagents are now offered in bundles with EPI64 Antibody (B-9) (see ordering information below).
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EPI64 Antibody (B-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects EPI64 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EPI64 (B-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EPI64, also known as EBP-PDZ interactor, plays a crucial role in cellular signaling by interacting with NHE regulatory factor 1 (NHERF-1) through its C-terminal -DTYL sequence, which binds to the first PDZ domain of NHERF-1 and NHERF-2. This interaction facilitates the regulation of Na+/H+ exchange protein (NHE), which is essential for transepithelial sodium absorption, primarily in intestinal and renal brush border membranes. By modulating NHE activity, EPI64 influences sodium homeostasis and blood pressure regulation, highlighting its importance in maintaining physiological balance. EPI64 is ubiquitously expressed and co-localizes with NHERF-1 in vitro, underscoring its role in cellular processes that are vital for proper organ function and overall health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
EPI64 Antibody (B-9) References:
- Identification of EPI64, a TBC/rabGAP domain-containing microvillar protein that binds to the first PDZ domain of EBP50 and E3KARP. | Reczek, D. and Bretscher, A. 2001. J Cell Biol. 153: 191-206. PMID: 11285285
- Identification of EPI64 as a GTPase-activating protein specific for Rab27A. | Itoh, T. and Fukuda, M. 2006. J Biol Chem. 281: 31823-31. PMID: 16923811
- EPI64 regulates microvillar subdomains and structure. | Hanono, A., et al. 2006. J Cell Biol. 175: 803-13. PMID: 17145964
- PDZs and receptor/channel clustering: rounding up the latest suspects. | Sheng, M. 1996. Neuron. 17: 575-8. PMID: 8893015
- The human testis determining factor SRY binds a nuclear factor containing PDZ protein interaction domains. | Poulat, F., et al. 1997. J Biol Chem. 272: 7167-72. PMID: 9054412
- cAMP-mediated inhibition of the epithelial brush border Na+/H+ exchanger, NHE3, requires an associated regulatory protein. | Yun, CH., et al. 1997. Proc Natl Acad Sci U S A. 94: 3010-5. PMID: 9096337
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EPI64 Antibody (B-9) | sc-376991 | 200 µg/ml | $322.00 | |||
EPI64 Antibody (B-9): m-IgG Fc BP-HRP Bundle | sc-527571 | 200 µg Ab; 10 µg BP | $361.00 | |||
EPI64 Antibody (B-9): m-IgG1 BP-HRP Bundle | sc-532944 | 200 µg Ab; 20 µg BP | $361.00 | |||
EPI64 Antibody (B-9) AC | sc-376991 AC | 500 µg/ml, 25% agarose | $424.00 | |||
EPI64 Antibody (B-9) HRP | sc-376991 HRP | 200 µg/ml | $322.00 | |||
EPI64 Antibody (B-9) FITC | sc-376991 FITC | 200 µg/ml | $336.00 | |||
EPI64 Antibody (B-9) PE | sc-376991 PE | 200 µg/ml | $349.00 | |||
EPI64 Antibody (B-9) Alexa Fluor® 488 | sc-376991 AF488 | 200 µg/ml | $364.00 | |||
EPI64 Antibody (B-9) Alexa Fluor® 546 | sc-376991 AF546 | 200 µg/ml | $364.00 | |||
EPI64 Antibody (B-9) Alexa Fluor® 594 | sc-376991 AF594 | 200 µg/ml | $364.00 | |||
EPI64 Antibody (B-9) Alexa Fluor® 647 | sc-376991 AF647 | 200 µg/ml | $364.00 | |||
EPI64 Antibody (B-9) Alexa Fluor® 680 | sc-376991 AF680 | 200 µg/ml | $364.00 | |||
EPI64 Antibody (B-9) Alexa Fluor® 790 | sc-376991 AF790 | 200 µg/ml | $364.00 |