Anti-ephrin-B3 Antibody (D-11) recommended for detection of ephrin-B3 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-ephrin-B3 Antibody (D-11): sc-390696

Anti-ephrin-B3 Antibody (D-11) is rated 4.0 out of 5 by 2.
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Datasheets

    • Anti-ephrin-B3 Antibody (D-11) is a mouse monoclonal IgG2a (kappa light chain) ephrin-B3 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 2-21 of ephrin-B3 of human origin
    • Anti-ephrin-B3 Antibody (D-11) is recommended for detection of ephrin-B3 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-ephrin-B3 Antibody (D-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ephrin-B3 (D-11): sc-390696.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for ephrin-B3 Antibody (D-11) for WB applications. These reagents are now offered in bundles with ephrin-B3 Antibody (D-11) (see ordering information below).
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What application is the blocking peptide sc-390696 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 3 out of 5 by from WB evaluation I tested this antibody with mouse brain cortex lysate. I got a band ~40-43kd, but I also got many other strong bands at ~23kd, ~50kd and a lot >70kd. So the specificity of this antibody was poor.
Date published: 2017-03-09
Rated 5 out of 5 by from Produced positive Western blot data of ephrin Produced positive Western blot data of ephrin-B3 expression in U-87 MG and Caki-1 whole cell lysates. -SCBT QC
Date published: 2013-03-30
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