Anti-ephrin-B3 Antibody (D-11) is a mouse monoclonal IgG2a (kappa light chain) ephrin-B3 antibody provided at 200 µg/ml
specific for an epitope mapping between amino acids 2-21 of ephrin-B3 of human origin
Anti-ephrin-B3 Antibody (D-11) is recommended for detection of ephrin-B3 of mouse, rat and human origin by WB, IP, IF and ELISA
Anti-ephrin-B3 Antibody (D-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ephrin-B3 (D-11): sc-390696.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
ephrin-B3 Antibody (D-11) is a high quality monoclonal ephrin-B3 antibody (also designated Ephrin B3 antibody or EFNB3 antibody) suitable for the detection of the ephrin-B3 protein of mouse, rat and human origin. ephrin-B3 Antibody (D-11) is available as both the non-conjugated anti-ephrin-B3 antibody form, as well as multiple conjugated forms of anti-ephrin-B3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The Eph subfamily represents the largest group of receptor protein kinases identified to date. There is increasing evidence that Eph family members are involved in central nervous system function and in development. Ligands for Eph receptors include ephrin-A1 (LERK-1/B61), identified as a ligand for the EphA2 (Eck)receptor; ephrin-A2 (ELF-1), identified as a ligand for the EphA3 and EphA4 (Sek) receptors; ephrin-A3 (LERK-3), identified as a ligand for EphA5 (Ehk1) and EphA3 (Hek) receptors; ephrin-A4 (LERK-4), identified as a ligand for the EphA3 receptor; ephrin-A5 (AL-1), identified as a ligand for EphA5 (REK7); ephrin-B1 (LERK-2), identified as a ligand for the EphB1 (Elk) and EphB2 (Cek5) receptors; ephrin-B2 (LERK-5), identified as a ligand for the EphB1, EphB3 (Cek10) and EphB2 receptors; and ephrin-B3 (LERK-8), identified as a ligand for EphB1.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What application is the blocking peptide sc-390696 P appropriate for?
Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here:
Rated 3 out of
Wan He from
WB evaluationI tested this antibody with mouse brain cortex lysate. I got a band ~40-43kd, but I also got many other strong bands at ~23kd, ~50kd and a lot >70kd. So the specificity of this antibody was poor.
Date published: 2017-03-09
Rated 5 out of
Produced positive Western blot data of ephrinProduced positive Western blot data of ephrin-B3 expression in U-87 MG and Caki-1 whole cell lysates. -SCBT QC
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