EphB4 Antibody (H-10): sc-365510

EphB4 Antibody (H-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects EphB4 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EphB4 Antibody (H-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EphB4 plays a crucial role in cell signaling and is involved in processes such as cell adhesion, migration, and differentiation, which are essential for normal development and function of the central nervous system. Notably, EphB4 interacts with ephrin-B2, a ligand that is significant for mediating bidirectional signaling pathways that influence cellular responses during developmental processes. The Eph receptor family, which includes EphB4, represents the largest group of receptor protein tyrosine kinases identified to date, and dysregulation has been implicated in various diseases, including cancer. Understanding the function and interactions of EphB4 is vital for elucidating its role in both physiological and pathological contexts, making anti-EphB4 antibody (H-10) an invaluable tool for researchers studying these processes.

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EphB4 Antibody (H-10) References:

1. EphB4 is overexpressed in gliomas and promotes the growth of glioma cells.  |  Chen, T., et al. 2013. Tumour Biol. 34: 379-85. PMID: 23138393
2. EphB4 promotes the proliferation, invasion, and angiogenesis of human colorectal cancer.  |  Lv, J., et al. 2016. Exp Mol Pathol. 100: 402-8. PMID: 27072105
3. EphB4-VAV1 signaling pathway is associated with imatinib resistance in chronic myeloid leukemia cells.  |  Zhang, JF., et al. 2016. Blood Cells Mol Dis. 59: 58-62. PMID: 27282569
4. Antitumor effects of circ-EPHB4 in hepatocellular carcinoma via inhibition of HIF-1α.  |  Tan, Y., et al. 2019. Mol Carcinog. 58: 875-886. PMID: 30644610
5. Angiogenesis depends upon EPHB4-mediated export of collagen IV from vascular endothelial cells.  |  Chen, D., et al. 2022. JCI Insight. 7: PMID: 35015735
6. Identification and cloning of ELF-1, a developmentally expressed ligand for the Mek4 and Sek receptor tyrosine kinases.  |  Cheng, HJ. and Flanagan, JG. 1994. Cell. 79: 157-68. PMID: 7522971
7. Molecular cloning of a ligand for the EPH-related receptor protein-tyrosine kinase Htk.  |  Bennett, BD., et al. 1995. Proc Natl Acad Sci U S A. 92: 1866-70. PMID: 7534404
8. Identification of full-length and truncated forms of Ehk-3, a novel member of the Eph receptor tyrosine kinase family.  |  Valenzuela, DM., et al. 1995. Oncogene. 10: 1573-80. PMID: 7731712
9. Identification of alternatively spliced mRNAs encoding variants of MDK1, a novel receptor tyrosine kinase expressed in the murine nervous system.  |  Ciossek, T., et al. 1995. Oncogene. 10: 97-108. PMID: 7824284
10. Ligands for the receptor tyrosine kinases hek and elk: isolation of cDNAs encoding a family of proteins.  |  Kozlosky, CJ., et al. 1995. Oncogene. 10: 299-306. PMID: 7838529
11. cDNA cloning and tissue distribution of five human EPH-like receptor protein-tyrosine kinases.  |  Fox, GM., et al. 1995. Oncogene. 10: 897-905. PMID: 7898931
12. Molecular characterization of a family of ligands for eph-related tyrosine kinase receptors.  |  Beckmann, MP., et al. 1994. EMBO J. 13: 3757-62. PMID: 8070404

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EphB4 Antibody (H-10) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-EphB4 Antibody (H-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EphB4 Antibody (H-10) Specifications

Alternate Product Name: EphB4

Product Clone Name: H-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 201-400

Epitope Target: EphB4

Epitope Species: human

Product Reactivity/Detection: EphB4

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for EphB4 Antibody (H-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin