EphA7 Antibody (E-7): sc-393973

EphA7 Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects EphA7 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EphA7 (E-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EphA7 protein, also known as ephrin type-A receptor 7, plays a crucial role in the nervous system by mediating axonal guidance during development. EphA7 is primarily located in the cell membrane, where EphA7 interacts with its ligands, the ephrins, to facilitate the repulsive signaling necessary for directing growing axons to their appropriate targets. This interaction is vital for establishing correct neural connections, as EphA7 ensures that axons do not stray from their intended pathways, which is essential for proper brain and spinal cord development. EphA7 undergoes alternative splicing, resulting in three different isoforms, including a full-length version and two truncated forms that lack kinase activity, which may have distinct roles in cellular signaling and development. Understanding EphA7 function and localization is important for elucidating contributions to neurodevelopmental processes and potential implications in neurological disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

EphA7 Antibody (E-7) References:

1. Regulation of repulsion versus adhesion by different splice forms of an Eph receptor.  |  Holmberg, J., et al. 2000. Nature. 408: 203-6. PMID: 11089974
2. Upregulation of EphA receptor expression in the injured adult rat spinal cord.  |  Willson, CA., et al. 2002. Cell Transplant. 11: 229-39. PMID: 12075988
3. EphA7-ephrin-A5 signaling in mouse somatosensory cortex: developmental restriction of molecular domains and postnatal maintenance of functional compartments.  |  Miller, K., et al. 2006. J Comp Neurol. 496: 627-42. PMID: 16615124
4. EphA5 and EphA7 forward signaling enhances human hematopoietic stem and progenitor cell maintenance, migration, and adhesion via Rac1 activation.  |  Nguyen, TM., et al. 2017. Exp Hematol. 48: 72-78. PMID: 27988259
5. EphA7 Functions as Receptor on BJAB Cells for Cell-to-Cell Transmission of the Kaposi's Sarcoma-Associated Herpesvirus and for Cell-Free Infection by the Related Rhesus Monkey Rhadinovirus.  |  Großkopf, AK., et al. 2019. J Virol. 93: PMID: 31118261
6. Circulating microRNA-944 and its target gene EPHA7 as a potential biomarker for colorectal cancer.  |  Shaker, OG., et al. 2022. Arch Physiol Biochem. 128: 1181-1187. PMID: 32421395
7. Mutually Repulsive EphA7-EfnA5 Organize Region-to-Region Corticopontine Projection by Inhibiting Collateral Extension.  |  Iguchi, T., et al. 2021. J Neurosci. 41: 4795-4808. PMID: 33906900
8. MicroRNA-146b-5p/EPHA7 axis regulates cell invasion, metastasis, proliferation, and temozolomide-induced chemoresistance via regulation of IRAK4/TRAF6/NF-κB signaling pathway in aggressive pituitary adenoma.  |  Lou, X., et al. 2022. Histol Histopathol. 37: 21-33. PMID: 34746996
9. c-Met and EPHA7 Receptor Tyrosine Kinases Are Related to Prognosis in Clear Cell Renal Cell Carcinoma: Focusing on the Association with Myoferlin Expression.  |  Jung, M., et al. 2022. Cancers (Basel). 14: PMID: 35205843
10. Identification of full-length and truncated forms of Ehk-3, a novel member of the Eph receptor tyrosine kinase family.  |  Valenzuela, DM., et al. 1995. Oncogene. 10: 1573-80. PMID: 7731712
11. Identification of alternatively spliced mRNAs encoding variants of MDK1, a novel receptor tyrosine kinase expressed in the murine nervous system.  |  Ciossek, T., et al. 1995. Oncogene. 10: 97-108. PMID: 7824284
12. cDNA cloning and tissue distribution of five human EPH-like receptor protein-tyrosine kinases.  |  Fox, GM., et al. 1995. Oncogene. 10: 897-905. PMID: 7898931

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EphA7 Antibody (E-7) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-EphA7 Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EphA7 Antibody (E-7) Specifications

Alternate Product Name: EphA7

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 39-62

Epitope Target: EphA7

Epitope Species: mouse

Product Reactivity/Detection: EphA7

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for EphA7 Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin