EphA5 Antibody (8B10B1): sc-517242

EphA5 Antibody (8B10B1) is a mouse monoclonal IgG1 kappa light chain antibody that detects EphA5 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-EphA5 antibody (8B10B1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EphA5, also known as ephrin type-A receptor 5, plays a crucial role in cell signaling and communication, particularly in the development of the central nervous system. EphA5 is primarily located on the cell surface, where EphA5 interacts with ephrin ligands to mediate bidirectional signaling pathways that influence cell positioning, migration, and differentiation. This interaction is vital for processes such as neuronal development and vascular formation, highlighting EphA5′s importance in both normal physiological functions and potential pathological conditions. The Eph receptor family, which includes EphA5, represents the largest group of receptor protein tyrosine kinases, and their diverse roles in cellular communication underscore their significance in developmental biology and disease mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

EphA5 Antibody (8B10B1) References:

1. EphA5 and EphA7 forward signaling enhances human hematopoietic stem and progenitor cell maintenance, migration, and adhesion via Rac1 activation.  |  Nguyen, TM., et al. 2017. Exp Hematol. 48: 72-78. PMID: 27988259
2. Dynamic Alterations of Retinal EphA5 Expression in Retinocollicular Map Plasticity.  |  Cheng, Q., et al. 2019. Dev Neurobiol. 79: 252-267. PMID: 30916472
3. EphA5 knockdown enhances the invasion and migration ability of esophageal squamous cell carcinoma via epithelial-mesenchymal transition through activating Wnt/β-catenin pathway.  |  Zhang, R., et al. 2020. Cancer Cell Int. 20: 20. PMID: 31956298
4. EPHA5 mutation impairs natural killer cell-mediated cytotoxicity against non-small lung cancer cells and promotes cancer cell migration and invasion.  |  Zhang, J., et al. 2020. Mol Cell Probes. 52: 101566. PMID: 32234341
5. EPHA5 Enhances the Stemness of Non-small cell lung cancer Cells Through Activating the Wnt Signaling Pathway.  |  Li, J. and Zhu, Y. 2021. J BUON. 26: 1871-1878. PMID: 34761594
6. Identification and cloning of ELF-1, a developmentally expressed ligand for the Mek4 and Sek receptor tyrosine kinases.  |  Cheng, HJ. and Flanagan, JG. 1994. Cell. 79: 157-68. PMID: 7522971
7. Molecular cloning of a ligand for the EPH-related receptor protein-tyrosine kinase Htk.  |  Bennett, BD., et al. 1995. Proc Natl Acad Sci U S A. 92: 1866-70. PMID: 7534404
8. Identification of full-length and truncated forms of Ehk-3, a novel member of the Eph receptor tyrosine kinase family.  |  Valenzuela, DM., et al. 1995. Oncogene. 10: 1573-80. PMID: 7731712
9. Identification of alternatively spliced mRNAs encoding variants of MDK1, a novel receptor tyrosine kinase expressed in the murine nervous system.  |  Ciossek, T., et al. 1995. Oncogene. 10: 97-108. PMID: 7824284
10. Ligands for the receptor tyrosine kinases hek and elk: isolation of cDNAs encoding a family of proteins.  |  Kozlosky, CJ., et al. 1995. Oncogene. 10: 299-306. PMID: 7838529
11. cDNA cloning and tissue distribution of five human EPH-like receptor protein-tyrosine kinases.  |  Fox, GM., et al. 1995. Oncogene. 10: 897-905. PMID: 7898931
12. Molecular characterization of a family of ligands for eph-related tyrosine kinase receptors.  |  Beckmann, MP., et al. 1994. EMBO J. 13: 3757-62. PMID: 8070404

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EphA5 Antibody (8B10B1) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-EphA5 Antibody (8B10B1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EphA5 Antibody (8B10B1) Specifications

Alternate Product Name: EphA5

Product Clone Name: 8B10B1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 620-774

Epitope Target: EphA5

Epitope Species: human

Product Reactivity/Detection: EphA5

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for EphA5 Antibody (8B10B1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin