EphA4 Antibody (D-4): sc-365503

EphA4 Antibody (D-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects EphA4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-EphA4 antibody (D-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EphA4 plays a crucial role in cell signaling and is involved in the regulation of cell adhesion, migration, and differentiation, particularly within the central nervous system. EphA4 functioning is essential for neural development and the establishment of synaptic connections, which are vital for cognitive processes and overall brain function. Dysregulation of EphA4 signaling has been implicated in various neurological disorders, making EphA4 a significant target for research in neurobiology and potential therapeutic interventions. The Eph subfamily of receptor protein tyrosine kinases, which includes EphA4, represents the largest group of such receptors identified to date, highlighting their importance in cellular communication and development. Ligands for Eph receptors, such as ephrin-A2, specifically bind to EphA4, further emphasizing the intricate signaling pathways that govern cellular interactions and responses.

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EphA4 Antibody (D-4) References:

1. The Role of EphA4 Signaling in Radiation-Induced EMT-Like Phenotype in Colorectal Cancer Cells.  |  de Marcondes, PG. and Morgado-Díaz, JA. 2017. J Cell Biochem. 118: 442-445. PMID: 27632701
2. EphA4 Obstructs Spinal Cord Neuron Regeneration by Promoting Excessive Activation of Astrocytes.  |  Chen, X., et al. 2022. Cell Mol Neurobiol. 42: 1557-1568. PMID: 33595805
3. Role of EphA4 in Mediating Motor Neuron Death in MND.  |  Zhao, J., et al. 2021. Int J Mol Sci. 22: PMID: 34502339
4. EphA4/ephrinA3 reverse signaling mediated downregulation of glutamate transporter GLAST in Müller cells in an experimental glaucoma model.  |  Zhou, ZX., et al. 2023. Glia. 71: 720-741. PMID: 36416239
5. MicroRNA-204-5p Ameliorates Neurological Injury via the EphA4/PI3K/AKT Signaling Pathway in Ischemic Stroke.  |  Shi, CS., et al. 2023. ACS Chem Neurosci. 14: 2060-2073. PMID: 37196241
6. Identification and cloning of ELF-1, a developmentally expressed ligand for the Mek4 and Sek receptor tyrosine kinases.  |  Cheng, HJ. and Flanagan, JG. 1994. Cell. 79: 157-68. PMID: 7522971
7. Molecular cloning of a ligand for the EPH-related receptor protein-tyrosine kinase Htk.  |  Bennett, BD., et al. 1995. Proc Natl Acad Sci U S A. 92: 1866-70. PMID: 7534404
8. Identification of full-length and truncated forms of Ehk-3, a novel member of the Eph receptor tyrosine kinase family.  |  Valenzuela, DM., et al. 1995. Oncogene. 10: 1573-80. PMID: 7731712
9. Identification of alternatively spliced mRNAs encoding variants of MDK1, a novel receptor tyrosine kinase expressed in the murine nervous system.  |  Ciossek, T., et al. 1995. Oncogene. 10: 97-108. PMID: 7824284
10. Ligands for the receptor tyrosine kinases hek and elk: isolation of cDNAs encoding a family of proteins.  |  Kozlosky, CJ., et al. 1995. Oncogene. 10: 299-306. PMID: 7838529
11. cDNA cloning and tissue distribution of five human EPH-like receptor protein-tyrosine kinases.  |  Fox, GM., et al. 1995. Oncogene. 10: 897-905. PMID: 7898931
12. Molecular characterization of a family of ligands for eph-related tyrosine kinase receptors.  |  Beckmann, MP., et al. 1994. EMBO J. 13: 3757-62. PMID: 8070404

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EphA4 Antibody (D-4) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-EphA4 Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EphA4 Antibody (D-4) Specifications

Alternate Product Name: EphA4

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 889-921

Epitope Target: EphA4

Epitope Species: human

Product Reactivity/Detection: EphA4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for EphA4 Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin