Epac Antibody (A-5) recommended for detection of Epac of mouse, rat, human and hamster origin by WB, IP, IF, IHC(P) and ELISA

Epac Antibody (A-5): sc-28366

Epac Antibody (A-5) is rated 4.4 out of 5 by 10.
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Datasheets

    • Anti-Epac Antibody (A-5) is a mouse monoclonal IgG2a κ Epac antibody, cited in 15 publications, provided at 200 µg/ml
    • raised against amino acids 1-70 of Epac of human origin
    • Anti-Epac Antibody (A-5) is recommended for detection of Epac of mouse, rat, human and hamster origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Epac Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Epac (A-5): sc-28366.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Epac Antibody (A-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with Epac Antibody (A-5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Can Epac (A-5): sc-28366 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Epac (A-5): sc-28366 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24

Does this recognize epac1 or epac2 or both?

Asked by: Marina1
Thank you for your question. This mouse monoclonal antibody, Epac (A-5): sc-28366, is raised against the gene product of RAPGEF3, also designated Epac or Epac1. The Uniprot accession number for the full length protein is O95398. You can find a description with links to NCBI for the gene and protein information on our product page, under the tab "Gene Info" next to "Description" about half way down the page (below the ordering information). https://www.scbt.com/scbt/product/epac-antibody-a-5
Answered by: Technical Support
Date published: 2016-11-21
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Rated 4 out of 5 by from Works well in bovine cell lysates I tested this antibody on bovine cell lysates and it works well. There are some smaller bands that show up maybe do to protein degradation.
Date published: 2017-10-04
Rated 5 out of 5 by from Great antibody It really worked nicely, shown nice bands at 100 kDa at 1:500 dilution.
Date published: 2017-04-09
Rated 5 out of 5 by from Beautiful results This antibody works well in standard lab applications and will provide great results in samples from several species. It has been published with several times.
Date published: 2017-01-27
Rated 5 out of 5 by from Worked better than sc Worked better than sc-25632 for WB using 293 cells that over-express protein.
Date published: 2015-09-24
Rated 4 out of 5 by from This monoclonal was better This monoclonal was better, detecting Epac, although its strongest signal is not Epac.
Date published: 2015-07-13
Rated 4 out of 5 by from Strong band in WB in Caki and CHO whole cell Strong band in WB in Caki and CHO whole cell lysates. -SCBT QC -SCBT QC
Date published: 2015-06-30
Rated 4 out of 5 by from Good publishable data in IHC and WB in human Good publishable data in IHC and WB in human TR4 cells. -SCBT Publication Review
Date published: 2015-05-30
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmic Produced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human salivary gland tissue. -SCBT QC
Date published: 2015-05-11
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