eotaxin CRISPR/Cas9 KO Plasmid (m): sc-422835
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eotaxin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the eotaxin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: eotaxin Antibody (C-3): sc-373767
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eotaxin CRISPR/Cas9 KO Plasmid (m) | sc-422835 | 20 µg | $397.00 |
Overview
Mouse Ccl11 encodes eotaxin, a CC chemokine that drives selective recruitment of eosinophils by binding CCR3 and shaping leukocyte trafficking in inflamed tissues. Eotaxin production by epithelial cells, fibroblasts, and immune cells promotes chemotaxis, adhesion, and tissue infiltration, integrating with cytokine networks such as IL-4/IL-13 signaling and downstream MAPK and NF-κB–regulated inflammatory programs. Ccl11 is widely studied in models of allergic airway inflammation, atopic dermatitis, and gastrointestinal eosinophilic inflammation, where eosinophil accumulation contributes to remodeling and barrier dysfunction. Beyond allergy, altered Ccl11/eotaxin signaling has been linked to neuroimmune crosstalk and age-associated inflammation, making it relevant for systems-level studies of chemokine-driven pathology.
eotaxin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ccl11 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ccl11 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ccl11 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish eotaxin protein expression.
This CRISPR knockout system enables efficient generation of Ccl11-deficient cell models for investigation of eotaxin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ccl11 exon(s) critical for eotaxin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ccl11 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by eotaxin CRISPR/Cas9 KO Plasmid (m) and eotaxin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ccl11 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by eotaxin HDR Plasmid (m) and eotaxin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ccl11 homology arms to support homology-directed repair at defined Ccl11 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.