ENX-2 Antibody (H-4): sc-515817

ENX-2 Antibody (H-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects ENX-2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ENX-2 (H-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ENX-2, a mammalian homolog of the Drosophila gene enhancer of zeste, plays a crucial role in chromatin regulation and is characterized by the presence of a SET domain, which is essential for histone methylation function. This post-translational modification influences gene expression by altering chromatin structure, thereby impacting cellular processes such as differentiation and development. ENX-2 is expressed ubiquitously in both adult and fetal tissues, suggesting importance in maintaining heterochromatin stability, which is critical for genomic integrity. Human ENX-2 is located on chromosome 17q21, and expression patterns indicate a significant role in the central and peripheral nervous systems, as well as in fetal hematopoiesis during development. Understanding ENX-2 function and regulation can provide insights into developmental biology and potential implications in diseases associated with chromatin dysregulation.

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ENX-2 Antibody (H-4) References:

1. Proliferative involvement of ENX-1, a putative human polycomb group gene, in haematopoietic cells.  |  Fukuyama, T., et al. 2000. Br J Haematol. 108: 842-7. PMID: 10792293
2. The bmi-1 and mel-18 gene products define a new family of DNA-binding proteins involved in cell proliferation and tumorigenesis.  |  Goebl, MG. 1991. Cell. 66: 623. PMID: 1823128
3. Isolation and developmental expression analysis of Enx-1, a novel mouse Polycomb group gene.  |  Hobert, O., et al. 1996. Mech Dev. 55: 171-84. PMID: 8861097
4. Characterization of EZH1, a human homolog of Drosophila Enhancer of zeste near BRCA1.  |  Abel, KJ., et al. 1996. Genomics. 37: 161-71. PMID: 8921387
5. Mammalian homologues of the Polycomb-group gene Enhancer of zeste mediate gene silencing in Drosophila heterochromatin and at S. cerevisiae telomeres.  |  Laible, G., et al. 1997. EMBO J. 16: 3219-32. PMID: 9214638
6. Interaction of mouse polycomb-group (Pc-G) proteins Enx1 and Enx2 with Eed: indication for separate Pc-G complexes.  |  van Lohuizen, M., et al. 1998. Mol Cell Biol. 18: 3572-9. PMID: 9584197
7. Characterization of interactions between the mammalian polycomb-group proteins Enx1/EZH2 and EED suggests the existence of different mammalian polycomb-group protein complexes.  |  Sewalt, RG., et al. 1998. Mol Cell Biol. 18: 3586-95. PMID: 9584199

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ENX-2 Antibody (H-4) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-ENX-2 Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ENX-2 Antibody (H-4) Specifications

Alternate Product Name: ENX-2

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 60-79

Epitope Target: ENX-2

Epitope Species: human

Product Reactivity/Detection: ENX-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for ENX-2 Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin