Endophilin II Antibody (A-11): sc-365704

Endophilin II Antibody (A-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects Endophilin II protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Endophilin II antibody (A-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Endophilin II (A-11) monoclonal antibody recognizes a protein that plays a crucial role in endocytosis, particularly in vesicle formation at the synapse, which is essential for neurotransmitter release and synaptic plasticity. Endophilin II protein is primarily located in presynaptic terminals of neurons, where Endophilin II interacts with other proteins such as amphiphysin and dynamin to facilitate vesicle budding from the plasma membrane. Endophilin II (A-11) antibody targets a protein vital for maintaining synaptic transmission and overall neuronal communication, making Endophilin II a significant focus of research in neurobiology and related fields. Endophilin II expression across various tissues, alongside Endophilin II interaction with other endophilins, underscores its importance in diverse cellular processes beyond the nervous system, including receptor signaling and membrane trafficking.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Endophilin II Antibody (A-11) References:

1. Endophilin I mediates synaptic vesicle formation by transfer of arachidonate to lysophosphatidic acid.  |  Schmidt, A., et al. 1999. Nature. 401: 133-41. PMID: 10490020
2. Identification of the endophilins (SH3p4/p8/p13) as novel binding partners for the beta1-adrenergic receptor.  |  Tang, Y., et al. 1999. Proc Natl Acad Sci U S A. 96: 12559-64. PMID: 10535961
3. The SH3 domains of endophilin and amphiphysin bind to the proline-rich region of synaptojanin 1 at distinct sites that display an unconventional binding specificity.  |  Cestra, G., et al. 1999. J Biol Chem. 274: 32001-7. PMID: 10542231
4. SH3-domain-containing proteins function at distinct steps in clathrin-coated vesicle formation.  |  Simpson, F., et al. 1999. Nat Cell Biol. 1: 119-24. PMID: 10559884
5. Activation of EGF receptor endocytosis and ERK1/2 signaling by BPGAP1 requires direct interaction with EEN/endophilin II and a functional RhoGAP domain.  |  Lua, BL. and Low, BC. 2005. J Cell Sci. 118: 2707-21. PMID: 15944398
6. The ubiquitin-like protein monoclonal nonspecific suppressor factor beta conjugates to endophilin II and regulates phagocytosis.  |  Nakamura, M. and Shimosaki, S. 2009. FEBS J. 276: 6355-63. PMID: 19796172
7. Endophilin B is required for the Drosophila oocyte to endocytose yolk downstream of Oskar.  |  Tsai, YC., et al. 2014. Development. 141: 563-73. PMID: 24401369
8. [The expression of Endophilin II and VEGF-C in human laryngeal cancer and connection with clinical treatment].  |  Yin, ZP. and Yang, C. 2015. Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 29: 1156-9. PMID: 26540913
9. Endophilin2 Interacts with GluA1 to Mediate AMPA Receptor Endocytosis Induced by Oligomeric Amyloid-β.  |  Zhang, J., et al. 2017. Neural Plast. 2017: 8197085. PMID: 28758034
10. A novel SH3-containing human gene family preferentially expressed in the central nervous system.  |  Giachino, C., et al. 1997. Genomics. 41: 427-34. PMID: 9169142
11. The SH3p4/Sh3p8/SH3p13 protein family: binding partners for synaptojanin and dynamin via a Grb2-like Src homology 3 domain.  |  Ringstad, N., et al. 1997. Proc Natl Acad Sci U S A. 94: 8569-74. PMID: 9238017
12. SH3 domain-dependent interactions of endophilin with amphiphysin.  |  Micheva, KD., et al. 1997. FEBS Lett. 414: 308-12. PMID: 9315708

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Endophilin II Antibody (A-11) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-Endophilin II Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Endophilin II Antibody (A-11) Specifications

Alternate Product Name: Endophilin II

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 256-315

Epitope Target: Endophilin II

Epitope Species: human

Product Reactivity/Detection: Endophilin II

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for Endophilin II Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin