Endophilin I Antibody (D-3): sc-48378

Endophilin I Antibody (D-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Endophilin I of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Endophilin I antibody (D-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The endophilin family consists of three SH3 domain-containing proteins—Endophilin I, II, and III—also known as SH3P4, SH3P8, and SH3P13, respectively. Endophilin I plays a crucial role in presynaptic vesicle trafficking at nerve terminals, associating with key proteins such as Amphiphysin, Synaptojanin, and Dynamin. Endophilin I is exclusively expressed in the brain, highlighting a specialized function in neuronal synapses, while Endophilin II and III are found in various tissues. Endophilin I is involved in modulating G protein-coupled receptor signaling, acting as an adapter protein that directs β1 adrenergic receptors to the endocytic machinery, which is vital for regulating neurotransmitter release and synaptic plasticity. This protein′s multifaceted role underscores its importance in both synaptic function and broader cellular signaling pathways.

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Endophilin I Antibody (D-3) References:

1. Endophilin I mediates synaptic vesicle formation by transfer of arachidonate to lysophosphatidic acid.  |  Schmidt, A., et al. 1999. Nature. 401: 133-41. PMID: 10490020
2. The SH3 domains of endophilin and amphiphysin bind to the proline-rich region of synaptojanin 1 at distinct sites that display an unconventional binding specificity.  |  Cestra, G., et al. 1999. J Biol Chem. 274: 32001-7. PMID: 10542231
3. SH3-domain-containing proteins function at distinct steps in clathrin-coated vesicle formation.  |  Simpson, F., et al. 1999. Nat Cell Biol. 1: 119-24. PMID: 10559884
4. Structure-based characterization of the binding of peptide to the human endophilin-1 Src homology 3 domain using position-dependent noncovalent potential analysis.  |  Fu, C., et al. 2012. J Mol Model. 18: 2153-61. PMID: 21947444
5. Proteome-wide inference of human endophilin 1-binding peptides.  |  Wu, G., et al. 2012. Protein Pept Lett. 19: 1094-102. PMID: 22512650
6. Endophilin-1 regulates blood-brain barrier permeability by controlling ZO-1 and occludin expression via the EGFR-ERK1/2 pathway.  |  Liu, W., et al. 2014. Brain Res. 1573: 17-26. PMID: 24854121
7. Endophilin-1 regulates blood-brain barrier permeability via EGFR-JNK signaling pathway.  |  Chen, L., et al. 2015. Brain Res. 1606: 44-53. PMID: 25721793
8. MicroRNA-107 prevents amyloid-beta induced blood-brain barrier disruption and endothelial cell dysfunction by targeting Endophilin-1.  |  Liu, W., et al. 2016. Exp Cell Res. 343: 248-257. PMID: 27038654
9. Endophilin 1 knockdown prevents synaptic dysfunction induced by oligomeric amyloid β.  |  Yin, Y., et al. 2019. Mol Med Rep. 19: 4897-4905. PMID: 31059028
10. A novel SH3-containing human gene family preferentially expressed in the central nervous system.  |  Giachino, C., et al. 1997. Genomics. 41: 427-34. PMID: 9169142
11. The SH3p4/Sh3p8/SH3p13 protein family: binding partners for synaptojanin and dynamin via a Grb2-like Src homology 3 domain.  |  Ringstad, N., et al. 1997. Proc Natl Acad Sci U S A. 94: 8569-74. PMID: 9238017
12. SH3 domain-dependent interactions of endophilin with amphiphysin.  |  Micheva, KD., et al. 1997. FEBS Lett. 414: 308-12. PMID: 9315708

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Endophilin I Antibody (D-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Endophilin I Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Endophilin I Antibody (D-3) Specifications

Alternate Product Name: Endophilin I

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 53-352

Epitope Target: Endophilin I

Epitope Species: human

Product Reactivity/Detection: Endophilin I

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Endophilin I Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin