endobrevin Antibody (G-12): sc-166820

endobrevin Antibody (G-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects endobrevin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-endobrevin antibody (G-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Endobrevin, also known as VAMP-8, is a 100 amino acid single-pass type IV membrane protein that plays a crucial role in exocytosis, which is essential for neurotransmitter release and cellular secretory pathways. Endobrevin is primarily located in the cytoplasmic membrane of cells, where endobrevin interacts with other proteins involved in vesicle fusion, such as soluble NSF attachment proteins (SNAPs) and N-ethylmaleimide-sensitive factor (NSF), forming a SNARE complex that facilitates docking and fusion of vesicles with target membranes. Proper functioning of endobrevin is vital for maintaining cellular communication and homeostasis, as disruptions in endobrevin activity can lead to various neurological disorders and impairments in secretion. anti-endobrevin antibody (G-12) serves as an invaluable tool for researchers studying mechanisms of exocytosis and endobrevin′s role in cellular processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

endobrevin Antibody (G-12) References:

1. Syntaxin 2 and endobrevin are required for the terminal step of cytokinesis in mammalian cells.  |  Low, SH., et al. 2003. Dev Cell. 4: 753-9. PMID: 12737809
2. Endobrevin/VAMP-8 is the primary v-SNARE for the platelet release reaction.  |  Ren, Q., et al. 2007. Mol Biol Cell. 18: 24-33. PMID: 17065550
3. Endobrevin/VAMP-8-dependent dense granule release mediates thrombus formation in vivo.  |  Graham, GJ., et al. 2009. Blood. 114: 1083-90. PMID: 19395672
4. The syntaxin family of vesicular transport receptors.  |  Bennett, MK., et al. 1993. Cell. 74: 863-73. PMID: 7690687
5. Synaptobrevin binding to synaptophysin: a potential mechanism for controlling the exocytotic fusion machine.  |  Edelmann, L., et al. 1995. EMBO J. 14: 224-31. PMID: 7835333
6. Synaptic core complex of synaptobrevin, syntaxin, and SNAP25 forms high affinity alpha-SNAP binding site.  |  McMahon, HT. and Südhof, TC. 1995. J Biol Chem. 270: 2213-7. PMID: 7836452
7. Exocytosis relating proteins in the nervous system.  |  Yamaguchi, K. and Akagawa, K. 1994. Neurosci Res. 20: 289-92. PMID: 7870381
8. Synaptic vesicle membrane fusion complex: action of clostridial neurotoxins on assembly.  |  Hayashi, T., et al. 1994. EMBO J. 13: 5051-61. PMID: 7957071
9. A role for synaptotagmin (p65) in regulated exocytosis.  |  Elferink, LA., et al. 1993. Cell. 72: 153-9. PMID: 8422678
10. Stimulation of NSF ATPase activity by alpha-SNAP is required for SNARE complex disassembly and exocytosis.  |  Barnard, RJ., et al. 1997. J Cell Biol. 139: 875-83. PMID: 9362506
11. Structural organization of the synaptic exocytosis core complex.  |  Lin, RC. and Scheller, RH. 1997. Neuron. 19: 1087-94. PMID: 9390521
12. Endobrevin, a novel synaptobrevin/VAMP-like protein preferentially associated with the early endosome.  |  Wong, SH., et al. 1998. Mol Biol Cell. 9: 1549-63. PMID: 9614193

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-endobrevin Antibody (G-12) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-endobrevin Antibody (G-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

endobrevin Antibody (G-12) Specifications

Alternate Product Name: endobrevin

Product Clone Name: G-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 28-47

Epitope Target: endobrevin

Epitope Species: human

Product Reactivity/Detection: endobrevin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for endobrevin Antibody (G-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin