Emt Antibody (3F227): sc-71041
- Emt Antibody (3F227) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 1-26 of Emt of mouse origin
- recommended for detection of Emt of mouse, rat and human origin by WB, IP and KA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Emt Antibody (3F227) for WB applications. These reagents are now offered in bundles with Emt Antibody (3F227) (see ordering information below).
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Emt Antibody (3F227) is a mouse monoclonal IgG1 antibody that detects Emt in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and kinase assays. Emt, also known as Itk or Tsk, plays a crucial role in T cell signaling and activation, which is vital for the immune response. Anti-Emt antibody (3F227) recognizes a protein primarily located in the cytoplasm that is essential for proper T cell functioning by mediating signaling pathways leading to T cell proliferation and differentiation. Emt′s cellular location enables interactions with various signaling molecules, influencing the activation of downstream pathways critical for effective immune responses. Additionally, Emt contains SH3 and SH2 domains, which facilitate interactions with other proteins, enhancing signal transduction. Emt monoclonal antibody (3F227) serves as an invaluable tool for researchers studying T cell biology and mechanisms underlying immune responses, providing insights into potential therapeutic targets for immune-related diseases.
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Emt Antibody (3F227) References:
- Itk/Emt/Tsk activation in response to CD3 cross-linking in Jurkat T cells requires ZAP-70 and Lat and is independent of membrane recruitment. | Shan, X. and Wange, RL. 1999. J Biol Chem. 274: 29323-30. PMID: 10506192
- ITK inhibitors in inflammation and immune-mediated disorders. | Sahu, N. and August, A. 2009. Curr Top Med Chem. 9: 690-703. PMID: 19689375
- Regulation of T-cell responses and disease by tec kinase Itk. | August, A. and Ragin, MJ. 2012. Int Rev Immunol. 31: 155-65. PMID: 22449075
- IL-2 Inducible Kinase ITK is Critical for HIV-1 Infection of Jurkat T-cells. | Hain, A., et al. 2018. Sci Rep. 8: 3217. PMID: 29453458
- Hierarchy of signaling thresholds downstream of the T cell receptor and the Tec kinase ITK. | Gallagher, MP., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 34452995
- Reciprocal SH2-SH3 Domain Contacts between ITK Molecules Limit T Cell Receptor Signaling in Th2-type CD4+ T Cells. | Chen, JL., et al. 2022. Immunol Invest. 51: 1612-1629. PMID: 34844506
- Interleukin-2-inducible T-cell kinase (Itk) signaling regulates potent noncanonical regulatory T cells. | Mammadli, M., et al. 2021. Clin Transl Med. 11: e625. PMID: 34919342
- ITK independent development of Th17 responses during hypersensitivity pneumonitis driven lung inflammation. | Elmore, J., et al. 2022. Commun Biol. 5: 162. PMID: 35210549
- Overcoming immune checkpoint blockade resistance in solid tumors with intermittent ITK inhibition. | Zhao, M., et al. 2023. Sci Rep. 13: 15678. PMID: 37735204
- The kinase ITK controls a Ca2+-mediated switch that balances TH17 and Treg cell differentiation. | Anannya, O., et al. 2024. Sci Signal. 17: eadh2381. PMID: 39042726
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Emt Antibody (3F227) | sc-71041 | 200 µg/ml | $322.00 | |||
Emt Antibody (3F227): m-IgG Fc BP-HRP Bundle | sc-539335 | 200 µg Ab; 10 µg BP | $361.00 | |||
Emt Antibody (3F227): m-IgG1 BP-HRP Bundle | sc-541418 | 200 µg Ab; 20 µg BP | $361.00 |