Emt Antibody (2F12): sc-23902

Emt Antibody (2F12) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Emt protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and kinase assays. Anti-Emt antibody (2F12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Emt protein, also known as Itk or Tsk, is a member of the Tec family of non-receptor tyrosine kinases, which plays a crucial role in T cell signaling and activation. Specifically, Emt protein is involved in the regulation of T cell receptor signaling pathways, which are essential for the immune response. Proper functioning of Emt protein is vital for T cell development and differentiation, as Emt helps modulate the activation of downstream signaling cascades that lead to T cell proliferation and cytokine production. Dysregulation of Emt protein can lead to impaired immune responses, making Emt a significant focus of research in immunology and potential therapeutic interventions. Emt (2F12) antibody is an invaluable tool for researchers studying T cell biology and the mechanisms underlying immune system function.

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Emt Antibody (2F12) References:

1. Treatment of Immunological or Inflammatory Disorders with ITK Kinase Inhibitors.  |  Abdel-Magid, AF. 2014. ACS Med Chem Lett. 5: 456-7. PMID: 24900859
2. BTK/ITK dual inhibitors: Modulating immunopathology and lymphopenia for COVID-19 therapy.  |  McGee, MC., et al. 2021. J Leukoc Biol. 109: 49-53. PMID: 32640487
3. Role of the IL-2 inducible tyrosine kinase ITK and its inhibitors in disease pathogenesis.  |  Lechner, KS., et al. 2020. J Mol Med (Berl). 98: 1385-1395. PMID: 32808093
4. Inherited human ITK deficiency impairs IFN-γ immunity and underlies tuberculosis.  |  Ogishi, M., et al. 2023. J Exp Med. 220: PMID: 36326697
5. Targeting kinase ITK treats autoimmune arthritis via orchestrating T cell differentiation and function.  |  Chen, Y., et al. 2023. Biomed Pharmacother. 169: 115886. PMID: 37992572
6. TNF-α signals through ITK-Akt-mTOR to drive CD4+ T cell metabolic reprogramming, which is dysregulated in rheumatoid arthritis.  |  Bishop, EL., et al. 2024. Sci Signal. 17: eadg5678. PMID: 38652761
7. CD28 is associated with and induces the immediate tyrosine phosphorylation and activation of the Tec family kinase ITK/EMT in the human Jurkat leukemic T-cell line.  |  August, A., et al. 1994. Proc Natl Acad Sci U S A. 91: 9347-51. PMID: 7524075
8. Identification of Rlk, a novel protein tyrosine kinase with predominant expression in the T cell lineage.  |  Hu, Q., et al. 1995. J Biol Chem. 270: 1928-34. PMID: 7829530
9. TXK, a novel human tyrosine kinase expressed in T cells shares sequence identity with Tec family kinases and maps to 4p12.  |  Haire, RN., et al. 1994. Hum Mol Genet. 3: 897-901. PMID: 7951233
10. BMX, a novel nonreceptor tyrosine kinase gene of the BTK/ITK/TEC/TXK family located in chromosome Xp22.2.  |  Tamagnone, L., et al. 1994. Oncogene. 9: 3683-8. PMID: 7970727
11. Colocalization of X-linked agammaglobulinemia and X-linked immunodeficiency genes.  |  Thomas, JD., et al. 1993. Science. 261: 355-8. PMID: 8332900
12. Structure and expression of novel protein-tyrosine kinases, Emb and Emt, in hematopoietic cells.  |  Yamada, N., et al. 1993. Biochem Biophys Res Commun. 192: 231-40. PMID: 8476425

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Emt Antibody (2F12) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Emt Antibody (2F12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Emt Antibody (2F12) Specifications

Alternate Product Name: Emt

Product Clone Name: 2F12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-26

Epitope Target: Emt

Epitope Species: mouse

Product Reactivity/Detection: Emt

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, KA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Emt Antibody (2F12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin