Elongin C Antibody (56) recommended for detection of Elongin C of mouse, rat and human origin by WB and IF

Elongin C Antibody (56): sc-135895

Elongin C Antibody (56) is rated 3.7 out of 5 by 3.
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Datasheets

    • Anti-Elongin C Antibody (56) is a mouse monoclonal IgG1 Elongin C antibody provided at 200 µg/ml
    • raised against amino acids 1-112 representing full length Elongin C of human origin
    • recommended for detection of Elongin C of mouse, rat and human origin by WB and IF; also reactive with additional species, including canine; not recommended for IP
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Elongin C (56): sc-135895.

What is the recommended fixation method for immunofluorescence staining with Elongin C (56): sc-135895 monoclonal antibody?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 3 out of 5 by from It is ok! I use this ab with 1:500 dilution for HCC cell lines (20ug). Band was weak but detectable at right size.
Date published: 2017-07-24
Rated 4 out of 5 by from Produced robust immunofluorescence nuclear Produced robust immunofluorescence nuclear and cytoplasmic staining in human fibroblast cells. -SCBT QC
Date published: 2015-03-26
Rated 4 out of 5 by from Good antibody for western Good antibody for western, clean background and specific band in Hep G2 whole cell lysate. - SCBT QC
Date published: 2015-01-10
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