Elmo1 Antibody (B-7): sc-271519
- Elmo1 Antibody (B-7) is a mouse monoclonal IgG1 κ Elmo1 antibody, cited in 5 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 37-71 near the N-terminus of Elmo1 of human origin
- Elmo1 Antibody (B-7) is recommended for detection of Elmo1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine and porcine
- Anti-Elmo1 Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Elmo1 Antibody (B-7) for WB applications. These reagents are now offered in bundles with Elmo1 Antibody (B-7) (see ordering information below).
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Elmo1 Antibody (B-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Elmo1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Elmo1 antibody (B-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Elmo1 plays a crucial role in regulating cell motility and phagocytosis, processes vital for immune response and tissue homeostasis. Elmo1 is primarily located in the cytoplasm, where Elmo1 interacts with other proteins such as DOCK180 and Crk, forming a complex essential for activating Rac1, a small GTPase involved in cytoskeletal rearrangements. This interaction is particularly important during apoptotic cell engulfment, as Elmo1 facilitates necessary changes in cell shape and movement. Src family kinase-mediated tyrosine phosphorylation of Elmo1 enhances signaling capabilities, thereby influencing various cellular processes. Elmo1 modulation of these pathways underscores Elmo1′s significance in both normal physiological functions and pathological conditions, making anti-Elmo1 antibody (B-7) a valuable tool for researchers studying cell motility and immune responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Elmo1 Antibody (B-7) References:
- CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration. | Gumienny, TL., et al. 2001. Cell. 107: 27-41. PMID: 11595183
- Unconventional Rac-GEF activity is mediated through the Dock180-ELMO complex. | Brugnera, E., et al. 2002. Nat Cell Biol. 4: 574-82. PMID: 12134158
- DOCK2 regulates Rac activation and cytoskeletal reorganization through interaction with ELMO1. | Sanui, T., et al. 2003. Blood. 102: 2948-50. PMID: 12829596
- RhoG activates Rac1 by direct interaction with the Dock180-binding protein Elmo. | Katoh, H. and Negishi, M. 2003. Nature. 424: 461-4. PMID: 12879077
- PH domain of ELMO functions in trans to regulate Rac activation via Dock180. | Lu, M., et al. 2004. Nat Struct Mol Biol. 11: 756-62. PMID: 15247908
- Phagocytosis of apoptotic cells is regulated by a UNC-73/TRIO-MIG-2/RhoG signaling module and armadillo repeats of CED-12/ELMO. | deBakker, CD., et al. 2004. Curr Biol. 14: 2208-16. PMID: 15620647
- C-terminal SH3 domain of CrkII regulates the assembly and function of the DOCK180/ELMO Rac-GEF. | Akakura, S., et al. 2005. J Cell Physiol. 204: 344-51. PMID: 15700267
- Identification of tyrosine residues on ELMO1 that are phosphorylated by the Src-family kinase Hck. | Yokoyama, N., et al. 2005. Biochemistry. 44: 8841-9. PMID: 15952790
- The DOCK180/Elmo complex couples ARNO-mediated Arf6 activation to the downstream activation of Rac1. | Santy, LC., et al. 2005. Curr Biol. 15: 1749-54. PMID: 16213822
- Neuronal adaptor FE65 stimulates Rac1-mediated neurite outgrowth by recruiting and activating ELMO1. | Li, W., et al. 2018. J Biol Chem. 293: 7674-7688. PMID: 29615491
- Knock-down of ELMO1 in Paediatric Rhabdomyosarcoma Cells by Nanoparticle Mediated siRNA Delivery. | Huang, X. and Townley, H. 2016. Nanobiomedicine (Rij). 3: 4. PMID: 29942379
- The crosstalk between microbial sensors ELMO1 and NOD2 shape intestinal immune responses. | Sharma, A., et al. 2023. Virulence. 14: 2171690. PMID: 36694274
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Elmo1 Antibody (B-7) | sc-271519 | 200 µg/ml | $316.00 | |||
Elmo1 Antibody (B-7): m-IgG Fc BP-HRP Bundle | sc-529200 | 200 µg Ab; 10 µg BP | $354.00 | |||
Elmo1 Antibody (B-7): m-IgGκ BP-HRP Bundle | sc-521967 | 200 µg Ab, 40 µg BP | $354.00 | |||
Elmo1 Antibody (B-7) AC | sc-271519 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Elmo1 Antibody (B-7) HRP | sc-271519 HRP | 200 µg/ml | $316.00 | |||
Elmo1 Antibody (B-7) FITC | sc-271519 FITC | 200 µg/ml | $330.00 | |||
Elmo1 Antibody (B-7) PE | sc-271519 PE | 200 µg/ml | $343.00 | |||
Elmo1 Antibody (B-7) Alexa Fluor® 488 | sc-271519 AF488 | 200 µg/ml | $357.00 | |||
Elmo1 Antibody (B-7) Alexa Fluor® 546 | sc-271519 AF546 | 200 µg/ml | $357.00 | |||
Elmo1 Antibody (B-7) Alexa Fluor® 594 | sc-271519 AF594 | 200 µg/ml | $357.00 | |||
Elmo1 Antibody (B-7) Alexa Fluor® 647 | sc-271519 AF647 | 200 µg/ml | $357.00 | |||
Elmo1 Antibody (B-7) Alexa Fluor® 680 | sc-271519 AF680 | 200 µg/ml | $357.00 | |||
Elmo1 Antibody (B-7) Alexa Fluor® 790 | sc-271519 AF790 | 200 µg/ml | $357.00 | |||
Elmo1 (B-7) Neutralizing Peptide | sc-271519 P | 100 µg/0.5 ml | $68.00 |