ELL2 Antibody (B-7): sc-515276

ELL2 Antibody (B-7) is a mouse monoclonal IgM antibody that detects ELL2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ELL2 antibody (B-7) is available as the non-conjugated form. ELL2, or RNA polymerase II elongation factor ELL2, is a crucial 640 amino acid nuclear protein that plays a significant role in gene expression regulation. ELL2 belongs to the ELL/occludin family, characterized by a highly conserved domain of approximately 100 amino acids found in all eukaryotic occludin proteins and RNA polymerase II elongation factors. ELL2 enhances the elongation phase of transcription by RNA polymerase II, effectively suppressing transient pausing at various sites along the DNA. ELL2 maintains transcription efficiency and fidelity by ensuring RNA polymerase II progresses smoothly along the DNA template. Additionally, ELL2 possesses a unique RNA polymerase II interaction domain that can negatively regulate polymerase activity during promoter-specific transcription initiation in vitro, highlighting a complex role in transcriptional regulation. ELL2 serves as an important target for studies related to gene expression and its dysregulation in various diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ELL2 Antibody (B-7) References:

1. EAF1, a novel ELL-associated factor that is delocalized by expression of the MLL-ELL fusion protein.  |  Simone, F., et al. 2001. Blood. 98: 201-9. PMID: 11418481
2. PITSLRE p110 protein kinases associate with transcription complexes and affect their activity.  |  Trembley, JH., et al. 2002. J Biol Chem. 277: 2589-96. PMID: 11709559
3. ELL-associated factor 2 (EAF2), a functional homolog of EAF1 with alternative ELL binding properties.  |  Simone, F., et al. 2003. Blood. 101: 2355-62. PMID: 12446457
4. Differential gene-expression profiling in the leukemia cell lines derived from indolent and aggressive phases of CD56+ T-cell large granular lymphocyte leukemia.  |  Daibata, M., et al. 2004. Int J Cancer. 108: 845-51. PMID: 14712486
5. Inhibition of the canonical Wnt signaling pathway in cytoplasm: a novel property of the carboxyl terminal domains of two Xenopus ELL genes.  |  Sakurai, K., et al. 2004. Zoolog Sci. 21: 407-16. PMID: 15118228
6. Noncanonical Wnt-4 signaling and EAF2 are required for eye development in Xenopus laevis.  |  Maurus, D., et al. 2005. EMBO J. 24: 1181-91. PMID: 15775981
7. Adrenal transcription regulatory genes modulated by angiotensin II and their role in steroidogenesis.  |  Romero, DG., et al. 2007. Physiol Genomics. 30: 26-34. PMID: 17327493
8. Increased phosphorylation of the carboxyl-terminal domain of RNA polymerase II and loading of polyadenylation and cotranscriptional factors contribute to regulation of the ig heavy chain mRNA in plasma cells.  |  Shell, SA., et al. 2007. J Immunol. 179: 7663-73. PMID: 18025212
9. Transcription elongation factor ELL2 directs immunoglobulin secretion in plasma cells by stimulating altered RNA processing.  |  Martincic, K., et al. 2009. Nat Immunol. 10: 1102-9. PMID: 19749764
10. Concurrent EAF2 and ELL2 loss phenocopies individual EAF2 or ELL2 loss in prostate cancer cells and murine prostate.  |  Zhong, M., et al. 2018. Am J Clin Exp Urol. 6: 234-244. PMID: 30697579
11. Host cell factors stimulate HIV-1 transcription by antagonizing substrate-binding function of Siah1 ubiquitin ligase to stabilize transcription elongation factor ELL2.  |  Wu, J., et al. 2020. Nucleic Acids Res. 48: 7321-7332. PMID: 32479599
12. Identification of a Nuclear Localization Signal (NLS) in Human Transcription Elongation Factor ELL2.  |  Kohrt, S., et al. 2024. Cell Biochem Funct. 42: e70019. PMID: 39582094

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ELL2 Antibody (B-7) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-ELL2 Antibody (B-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ELL2 Antibody (B-7) Specifications

Alternate Product Name: ELL2

Product Clone Name: B-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 19-36

Epitope Target: ELL2

Epitope Species: human

Product Reactivity/Detection: ELL2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for ELL2 Antibody (B-7): P: Blocking Peptide, X: TransCruz Reagent