Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Elk-1 CRISPR/Cas9 KO Plasmid (m): sc-420156
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Elk-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Elk-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: p-Elk-1 Antibody (B-4): sc-8406
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Elk-1 CRISPR/Cas9 KO Plasmid (m) | sc-420156 | 20 µg | $397.00 |
Overview
Elk1 encodes Elk-1, an ETS-domain transcription factor that functions as a nuclear effector of MAPK/ERK signaling. Upon phosphorylation by ERK, JNK, or p38 kinases, Elk-1 cooperates with serum response factor (SRF) at serum response elements to regulate immediate-early gene programs such as FOS and EGR family members. This activity links extracellular cues to changes in proliferation, differentiation, neuronal plasticity, and stress-responsive transcription in mouse cells. Dysregulated Elk-1–dependent transcription has been studied in contexts involving altered mitogenic signaling, inflammation-associated gene expression, and neurodegeneration-relevant pathways, making it a useful node for pathway interrogation.
Elk-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Elk1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Elk1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Elk1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Elk-1 protein expression.
This CRISPR knockout system enables efficient generation of Elk1-deficient cell models for investigation of Elk-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Elk1 exon(s) critical for Elk-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Elk1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Elk-1 CRISPR/Cas9 KO Plasmid (m) and Elk-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Elk1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Elk-1 HDR Plasmid (m) and Elk-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Elk1 homology arms to support homology-directed repair at defined Elk1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.