p-Elk-1 Antibody (B-4): sc-8406

p-Elk-1 Antibody (B-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects p-Elk-1 phosphorylated at Ser 383 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p-Elk-1 (B-4) antibody is available in non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. p-Elk-1 plays a crucial role in cellular signaling pathways, particularly in regulating gene expression in response to mitogenic stimuli. p-Elk-1 is a 428 amino acid nuclear protein belonging to the Ets family, characterized by a highly conserved carboxy-terminal domain essential for DNA binding. This domain facilitates p-Elk-1 interaction with specific purine-rich DNA sequences and influences transcriptional activity. Upon mitogenic stimulation, p-Elk-1 undergoes phosphorylation by mitogen-activated protein kinase 1, critical for function as a transcriptional activator. Phosphorylation leads to SUMOylation release, a post-translational modification that otherwise recruits histone deacetylase 2 to target gene promoters, resulting in decreased histone acetylation and diminished transactivator activity. p-Elk-1′s ability to modulate gene expression highlights significance in various biological processes, including cell proliferation and differentiation, making p-Elk-1 a valuable target for research in cancer and developmental biology.

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p-Elk-1 Antibody (B-4) References:

1. Molecular cloning and characterization of PEA3, a new member of the Ets oncogene family that is differentially expressed in mouse embryonic cells.  |  Xin, JH., et al. 1992. Genes Dev. 6: 481-96. PMID: 1547944
2. Elk-1, CREB, and MKP-1 regulate Egr-1 expression in gonadotropin-releasing hormone stimulated gonadotrophs.  |  Mayer, SI., et al. 2008. J Cell Biochem. 105: 1267-78. PMID: 18814180
3. The Drosophila 74EF early puff contains E74, a complex ecdysone-inducible gene that encodes two ets-related proteins.  |  Burtis, KC., et al. 1990. Cell. 61: 85-99. PMID: 2107982
4. elk, tissue-specific ets-related genes on chromosomes X and 14 near translocation breakpoints.  |  Rao, VN., et al. 1989. Science. 244: 66-70. PMID: 2539641
5. Identification and preferential expression in thymic and bursal lymphocytes of a c-ets oncogene-encoded Mr 54,000 cytoplasmic protein.  |  Ghysdael, J., et al. 1986. Proc Natl Acad Sci U S A. 83: 1714-8. PMID: 3006066
6. ELK-1 ubiquitination status and transcriptional activity are modulated independently of F-Box protein FBXO25.  |  Quintero-Barceinas, RS., et al. 2021. J Biol Chem. 296: 100214. PMID: 33428929
7. Critical Protein-Protein Interactions Determine the Biological Activity of Elk-1, a Master Regulator of Stimulus-Induced Gene Transcription.  |  Thiel, G., et al. 2021. Molecules. 26: PMID: 34684708
8. Asprosin inhibits macrophage lipid accumulation and reduces atherosclerotic burden by up-regulating ABCA1 and ABCG1 expression via the p38/Elk-1 pathway.  |  Zou, J., et al. 2022. J Transl Med. 20: 337. PMID: 35902881
9. Elk-1 regulates retinal ganglion cell axon regeneration after injury.  |  Noro, T., et al. 2022. Sci Rep. 12: 17446. PMID: 36261683
10. The Ets1 transcription factor is widely expressed during murine embryo development and is associated with mesodermal cells involved in morphogenetic processes such as organ formation.  |  Kola, I., et al. 1993. Proc Natl Acad Sci U S A. 90: 7588-92. PMID: 7689222
11. Differentially spliced erg-3 product functions as a transcriptional activator.  |  Prasad, DD., et al. 1994. Oncogene. 9: 669-73. PMID: 8290279
12. Effect of PU.1 phosphorylation on interaction with NF-EM5 and transcriptional activation.  |  Pongubala, JM., et al. 1993. Science. 259: 1622-5. PMID: 8456286

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Elk-1 Antibody (B-4) has 89 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Elk-1 Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Elk-1 Antibody (B-4) Specifications

Alternate Product Name: Elk-1

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 383

Epitope Target: Elk-1

Epitope Species: human

Product Reactivity/Detection: p-Elk-1 phosphorylated at Ser 383

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 89 citations in various scientific publications

Available Conjugates/Forms for p-Elk-1 Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent