Elk-1 Antibody (E-5): sc-365876

Elk-1 Antibody (E-5) is a mouse monoclonal IgG2b kappa light chain antibody designed to detect Elk-1, also known as ETS transcription factor ELK1 or SAP-2, across human, mouse, and rat species. Elk-1 is a pivotal member of the Ets family of transcription factors, characterized by its distinct ETS DNA-binding domain that facilitates binding to specific DNA motifs, particularly those containing the 5′-GGAA-3′ sequence. Anti-Elk-1 antibody (E-5) targets an internal region of Elk-1, specifically amino acids 161-320, ensuring precise recognition and binding to functional domains. The structural integrity of Elk-1 is crucial for regulating gene expression related to cell proliferation, differentiation, and response to extracellular signals, particularly through the MAPK/ERK signaling pathway. Anti-Elk-1 antibody (E-5) effectively aids in elucidating Elk-1 localization and interaction within the nucleus, where transcriptional activity occurs. Elk-1′s ability to form complexes with other transcription factors and co-activators, such as CBP/p300, is essential for the modulation of immediate early genes, such as c-Fos, which are vital for various cellular processes including growth and oncogenesis. Elk-1 monoclonal antibody (E-5) is validated for multiple applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), providing versatile tools for researchers to investigate Elk-1′s structural and functional dynamics. Anti-Elk-1 antibody (E-5) facilitates a deeper understanding of Elk-1′s role in signaling pathways and implications in disease mechanisms, thereby supporting advancements in targeted therapeutic development and molecular biology research.

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Elk-1 Antibody (E-5) References:

1. Molecular cloning and characterization of PEA3, a new member of the Ets oncogene family that is differentially expressed in mouse embryonic cells.  |  Xin, JH., et al. 1992. Genes Dev. 6: 481-96. PMID: 1547944
2. The Drosophila 74EF early puff contains E74, a complex ecdysone-inducible gene that encodes two ets-related proteins.  |  Burtis, KC., et al. 1990. Cell. 61: 85-99. PMID: 2107982
3. Elk-1 a transcription factor with multiple facets in the brain.  |  Besnard, A., et al. 2011. Front Neurosci. 5: 35. PMID: 21441990
4. Sequential activation of Elk-1/Egr-1/GADD45α by arsenic.  |  Shi, Q., et al. 2014. Oncotarget. 5: 3862-70. PMID: 25004251
5. elk, tissue-specific ets-related genes on chromosomes X and 14 near translocation breakpoints.  |  Rao, VN., et al. 1989. Science. 244: 66-70. PMID: 2539641
6. MZF-1/Elk-1 interaction domain as therapeutic target for protein kinase Cα-based triple-negative breast cancer cells.  |  Lee, CJ., et al. 2016. Oncotarget. 7: 59845-59859. PMID: 27542222
7. Identification and preferential expression in thymic and bursal lymphocytes of a c-ets oncogene-encoded Mr 54,000 cytoplasmic protein.  |  Ghysdael, J., et al. 1986. Proc Natl Acad Sci U S A. 83: 1714-8. PMID: 3006066
8. Critical Protein-Protein Interactions Determine the Biological Activity of Elk-1, a Master Regulator of Stimulus-Induced Gene Transcription.  |  Thiel, G., et al. 2021. Molecules. 26: PMID: 34684708
9. Glucose Homeostasis and Pancreatic Islet Size Are Regulated by the Transcription Factors Elk-1 and Egr-1 and the Protein Phosphatase Calcineurin.  |  Thiel, G. and Rössler, OG. 2023. Int J Mol Sci. 24: PMID: 36614256
10. The Ets1 transcription factor is widely expressed during murine embryo development and is associated with mesodermal cells involved in morphogenetic processes such as organ formation.  |  Kola, I., et al. 1993. Proc Natl Acad Sci U S A. 90: 7588-92. PMID: 7689222
11. Differentially spliced erg-3 product functions as a transcriptional activator.  |  Prasad, DD., et al. 1994. Oncogene. 9: 669-73. PMID: 8290279
12. Effect of PU.1 phosphorylation on interaction with NF-EM5 and transcriptional activation.  |  Pongubala, JM., et al. 1993. Science. 259: 1622-5. PMID: 8456286

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Elk-1 Antibody (E-5) has 27 citations in a variety of scientific publications.

Primary antibodies like Anti-Elk-1 Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Elk-1 Antibody (E-5) Specifications

Alternate Product Name: Elk-1

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 161-320

Epitope Target: Elk-1

Epitope Species: human

Product Reactivity/Detection: Elk-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 27 citations in various scientific publications

Available Conjugates/Forms for Elk-1 Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred p-Elk-1 antibody is p-Elk-1 Antibody (B-4): sc-8406.