eIF5A Antibody (H-8): sc-390202

eIF5A Antibody (H-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects eIF5A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-eIF5A antibody (H-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. eIF5A plays a crucial role in cellular processes, particularly in translation initiation, where eIF5A is essential for proper functioning of ribosomal peptidyl-transferase. eIF5A is encoded by the human eukaryotic translation initiation factor 5A gene, also known as eIF-4D or eIF5A1, which is located on chromosome 17p13 and produces a 154 amino acid protein vital for maintaining cellular polyamine homeostasis. eIF5A1 is localized in both nuclear and cytoplasmic compartments of mammalian cells, where eIF5A1 not only stimulates translation but also may facilitate nucleocytoplasmic mRNA transport. A unique aspect of eIF5A1 is post-translational modification at Lys-50, where hypusine is formed, a modification critical for biochemical activity and necessary for cellular proliferative signaling. Furthermore, eIF5A1 serves as a cofactor for Rev transactivator protein of human immunodeficiency virus type 1 (HIV-1), and disruption of eIF5A1 interaction with Rev can inhibit viral replication cycle. Additionally, eIF5A2, a highly conserved protein with 94% sequence similarity to eIF5A1, is implicated in cancer biology, as eIF5A2 gene amplification is observed in ovarian carcinomas, linking eIF5A2 overexpression to advanced stages of ovarian cancer.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

eIF5A Antibody (H-8) References:

1. Human eIF5A2 on chromosome 3q25-q27 is a phylogenetically conserved vertebrate variant of eukaryotic translation initiation factor 5A with tissue-specific expression.  |  Jenkins, ZA., et al. 2001. Genomics. 71: 101-9. PMID: 11161802
2. Isolation of a novel candidate oncogene within a frequently amplified region at 3q26 in ovarian cancer.  |  Guan, XY., et al. 2001. Cancer Res. 61: 3806-9. PMID: 11325856
3. Identification and characterization of eukaryotic initiation factor 5A-2.  |  Clement, PM., et al. 2003. Eur J Biochem. 270: 4254-63. PMID: 14622290
4. Mechanism and regulation of eukaryotic protein synthesis.  |  Merrick, WC. 1992. Microbiol Rev. 56: 291-315. PMID: 1620067
5. Differential expression of eIF5A-1 and eIF5A-2 in human cancer cells.  |  Clement, PM., et al. 2006. FEBS J. 273: 1102-14. PMID: 16519677
6. Translational control in mammalian cells.  |  Hershey, JW. 1991. Annu Rev Biochem. 60: 717-55. PMID: 1883206
7. Sequence determination and cDNA cloning of eukaryotic initiation factor 4D, the hypusine-containing protein.  |  Smit-McBride, Z., et al. 1989. J Biol Chem. 264: 1578-83. PMID: 2492279
8. The translation factor eIF5A and human cancer.  |  Mathews, MB. and Hershey, JW. 2015. Biochim Biophys Acta. 1849: 836-44. PMID: 25979826
9. Molecular basis of eIF5A-dependent CAT tailing in eukaryotic ribosome-associated quality control.  |  Tesina, P., et al. 2023. Mol Cell. 83: 607-621.e4. PMID: 36804914
10. P53-dependent hypusination of eIF5A affects mitochondrial translation and senescence immune surveillance.  |  Jiang, X., et al. 2024. Nat Commun. 15: 7458. PMID: 39198484
11. The eukaryotic cofactor for the human immunodeficiency virus type 1 (HIV-1) rev protein, eIF-5A, maps to chromosome 17p12-p13: three eIF-5A pseudogenes map to 10q23.3, 17q25, and 19q13.2.  |  Steinkasserer, A., et al. 1995. Genomics. 25: 749-52. PMID: 7759117
12. Induced gene expression of the hypusine-containing protein eukaryotic initiation factor 5A in activated human T lymphocytes.  |  Bevec, D., et al. 1994. Proc Natl Acad Sci U S A. 91: 10829-33. PMID: 7971969

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-eIF5A Antibody (H-8) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-eIF5A Antibody (H-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

eIF5A Antibody (H-8) Specifications

Alternate Product Name: eIF5A

Product Clone Name: H-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 9-124

Epitope Target: eIF5A1

Epitope Species: human

Product Reactivity/Detection: eIF5A1 and eIF5A2 of mouse, rat and human origin, and eIF5AL1 of human origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for eIF5A Antibody (H-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin