eIF4AI/II Antibody (H-5): sc-377315

eIF4AI/II Antibody (H-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects eIF4AI/II protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-eIF4AI/II antibody (H-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. eIF4AI/II protein plays a crucial role in translation initiation in eukaryotic cells, where eIF4AI/II functions as a key component of the eIF4F complex, essential for 80S ribosomal complex assembly. This complex binds to the 5′-cap of mRNA and facilitates 43S ribosomal preinitiation complex recruitment, ensuring efficient protein synthesis. eIF4AI/II interaction with other eukaryotic initiation factors, such as eIF4E and eIF4G, is vital for unwinding mRNA secondary structures, allowing proper ribosome association and translation initiation. Human eIF4AI (also known as DDX2A) consists of 406 amino acids and exhibits high homology (92.7%) with mouse eIF4AI, indicating conserved functional roles across species. Human eIF4A1 promoter region contains critical regulatory elements, including TATA and CAAT motifs, as well as consensus binding sites for transcription factors such as Sp1 and AP2, highlighting eIF4AI/II importance in gene expression regulation and cellular function.

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eIF4AI/II Antibody (H-5) References:

1. eIF4 initiation factors: effectors of mRNA recruitment to ribosomes and regulators of translation.  |  Gingras, AC., et al. 1999. Annu Rev Biochem. 68: 913-63. PMID: 10872469
2. A multifactor complex of eukaryotic initiation factors, eIF1, eIF2, eIF3, eIF5, and initiator tRNA(Met) is an important translation initiation intermediate in vivo.  |  Asano, K., et al. 2000. Genes Dev. 14: 2534-46. PMID: 11018020
3. Cleavage of translation initiation factor 4AI (eIF4AI) but not eIF4AII by foot-and-mouth disease virus 3C protease: identification of the eIF4AI cleavage site.  |  Li, W., et al. 2001. FEBS Lett. 507: 1-5. PMID: 11682048
4. Interactions between eIF4AI and its accessory factors eIF4B and eIF4H.  |  Rozovsky, N., et al. 2008. RNA. 14: 2136-48. PMID: 18719248
5. Bidirectional RNA helicase activity of eucaryotic translation initiation factors 4A and 4F.  |  Rozen, F., et al. 1990. Mol Cell Biol. 10: 1134-44. PMID: 2304461
6. Synthesis of biotinylated episilvestrol: highly selective targeting of the translation factors eIF4AI/II.  |  Chambers, JM., et al. 2013. Org Lett. 15: 1406-9. PMID: 23461621
7. Targeting the N Terminus of eIF4AI for Inhibition of Its Catalytic Recycling.  |  Jiang, C., et al. 2019. Cell Chem Biol. 26: 1417-1426.e5. PMID: 31402318
8. The knockdown of eIF4AI interferes with the respiratory syncytial virus replication cycle.  |  Martínez-Martínez, N., et al. 2021. Acta Virol. 65: 433-439. PMID: 34978844
9. Initiation of protein synthesis in eukaryotic cells.  |  Pain, VM. 1996. Eur J Biochem. 236: 747-71. PMID: 8665893
10. Characterization of the cloned promoter of the human initiation factor 4AI gene.  |  Kukimoto, I., et al. 1997. Biochem Biophys Res Commun. 233: 844-7. PMID: 9168945
11. A novel functional human eukaryotic translation initiation factor 4G.  |  Gradi, A., et al. 1998. Mol Cell Biol. 18: 334-42. PMID: 9418880
12. A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation.  |  Imataka, H., et al. 1998. EMBO J. 17: 7480-9. PMID: 9857202

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-eIF4AI/II Antibody (H-5) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-eIF4AI/II Antibody (H-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

eIF4AI/II Antibody (H-5) Specifications

Alternate Product Name: eIF4AI/II

Product Clone Name: H-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 186-330

Epitope Target: eIF4AI

Epitope Species: human

Product Reactivity/Detection: eIF4AI and eIF4AII

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for eIF4AI/II Antibody (H-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred eIF4AII antibody is eIF4AII Antibody (H-5): sc-137148.