eIF4AII Antibody (H-5): sc-137148

eIF4AII Antibody (H-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects eIF4AII in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-eIF4AII antibody (H-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. eIF4AII plays a crucial role in translation initiation, which is essential for protein synthesis in eukaryotic cells. eIF4AII functions as a component of the eIF4F complex, which binds to the 5′ cap of mRNA and facilitates the recruitment of the ribosomal preinitiation complex, ensuring efficient translation. eIF4AII helps unwind mRNA secondary structures, allowing ribosomes to access the mRNA and initiate translation. eIF4AII also interacts with other key proteins in the translation initiation process, including eIF4E and eIF4G, which are critical for the assembly of the translation machinery. Understanding eIF4AII dynamics and interactions helps elucidate the mechanisms of gene expression regulation and potential implications in various diseases, including cancer, where translation initiation is often dysregulated.

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eIF4AII Antibody (H-5) References:

1. eIF4 initiation factors: effectors of mRNA recruitment to ribosomes and regulators of translation.  |  Gingras, AC., et al. 1999. Annu Rev Biochem. 68: 913-63. PMID: 10872469
2. A multifactor complex of eukaryotic initiation factors, eIF1, eIF2, eIF3, eIF5, and initiator tRNA(Met) is an important translation initiation intermediate in vivo.  |  Asano, K., et al. 2000. Genes Dev. 14: 2534-46. PMID: 11018020
3. Translational repression and eIF4A2 activity are critical for microRNA-mediated gene regulation.  |  Meijer, HA., et al. 2013. Science. 340: 82-5. PMID: 23559250
4. eIF4AII is dispensable for miRNA-mediated gene silencing.  |  Galicia-Vázquez, G., et al. 2015. RNA. 21: 1826-33. PMID: 26286746
5. eIF4A2 targets developmental potency and histone H3.3 transcripts for translational control of stem cell pluripotency.  |  Li, D., et al. 2022. Sci Adv. 8: eabm0478. PMID: 35353581
6. Rare EIF4A2 variants are associated with a neurodevelopmental disorder characterized by intellectual disability, hypotonia, and epilepsy.  |  Paul, MS., et al. 2023. Am J Hum Genet. 110: 548. PMID: 36868207
7. Isolation and mapping of the human EIF4A2 gene homologous to the murine protein synthesis initiation factor 4A-II gene Eif4a2.  |  Sudo, K., et al. 1995. Cytogenet Cell Genet. 71: 385-8. PMID: 8521730
8. Initiation of protein synthesis in eukaryotic cells.  |  Pain, VM. 1996. Eur J Biochem. 236: 747-71. PMID: 8665893
9. Characterization of the cloned promoter of the human initiation factor 4AI gene.  |  Kukimoto, I., et al. 1997. Biochem Biophys Res Commun. 233: 844-7. PMID: 9168945
10. The role in neural patterning of translation initiation factor eIF4AII; induction of neural fold genes.  |  Morgan, R. and Sargent, MG. 1997. Development. 124: 2751-60. PMID: 9226446
11. A novel functional human eukaryotic translation initiation factor 4G.  |  Gradi, A., et al. 1998. Mol Cell Biol. 18: 334-42. PMID: 9418880
12. A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation.  |  Imataka, H., et al. 1998. EMBO J. 17: 7480-9. PMID: 9857202

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-eIF4AII Antibody (H-5) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-eIF4AII Antibody (H-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

eIF4AII Antibody (H-5) Specifications

Alternate Product Name: eIF4AII

Product Clone Name: H-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 2-43

Epitope Target: eIF4AII

Epitope Species: human

Product Reactivity/Detection: eIF4AII

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for eIF4AII Antibody (H-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin