eIF3ζ Antibody (H-7): sc-271515
- eIF3ζ Antibody (H-7) is a mouse monoclonal IgG1 κ eIF3ζ antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of eIF3ζ of human origin
- eIF3 zeta Antibody (H-7) is recommended for detection of eIF3ζ of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-eIF3 zeta Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for eIF3ζ Antibody (H-7) for WB applications. These reagents are now offered in bundles with eIF3ζ Antibody (H-7) (see ordering information below).
eIF3ζ Antibody (H-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects eIF3 zeta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). eIF3ζ (H-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. eIF3 zeta protein plays a crucial role in the initiation of protein synthesis, acting as a key component of the eukaryotic initiation factor 3 (eIF3) complex, which is essential for the assembly of the 40S ribosomal subunit. This complex facilitates the recruitment of the mRNA and the initiator tRNA, thereby ensuring accurate translation initiation. Proper functioning of eIF3 zeta is vital for cellular homeostasis, as dysregulation of protein synthesis can lead to various diseases, including cancer. Notably, overexpression of eIF3 proteins, including eIF3 zeta, has been implicated in tumorigenesis, highlighting their potential as therapeutic targets. Using anti-eIF3ζ antibody (H-7) in research can provide valuable insights into the mechanisms of protein synthesis and its implications in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
eIF3ζ Antibody (H-7) References:
- Interactions of eukaryotic translation initiation factor 3 (eIF3) subunit NIP1/c with eIF1 and eIF5 promote preinitiation complex assembly and regulate start codon selection. | Valásek, L., et al. 2004. Mol Cell Biol. 24: 9437-55. PMID: 15485912
- eIF3: a connecTOR of S6K1 to the translation preinitiation complex. | Peterson, TR. and Sabatini, DM. 2005. Mol Cell. 20: 655-7. PMID: 16337588
- Translation initiation factor eIF4G-1 binds to eIF3 through the eIF3e subunit. | LeFebvre, AK., et al. 2006. J Biol Chem. 281: 22917-32. PMID: 16766523
- Initiation factor eIF3 and regulation of mRNA translation, cell growth, and cancer. | Dong, Z. and Zhang, JT. 2006. Crit Rev Oncol Hematol. 59: 169-80. PMID: 16829125
- eIF3: a versatile scaffold for translation initiation complexes. | Hinnebusch, AG. 2006. Trends Biochem Sci. 31: 553-62. PMID: 16920360
- Individual overexpression of five subunits of human translation initiation factor eIF3 promotes malignant transformation of immortal fibroblast cells. | Zhang, L., et al. 2007. J Biol Chem. 282: 5790-800. PMID: 17170115
- Reconstitution reveals the functional core of mammalian eIF3. | Masutani, M., et al. 2007. EMBO J. 26: 3373-83. PMID: 17581632
- Measles virus N protein inhibits host translation by binding to eIF3-p40. | Sato, H., et al. 2007. J Virol. 81: 11569-76. PMID: 17686838
- An oncogenic role for the phosphorylated h-subunit of human translation initiation factor eIF3. | Zhang, L., et al. 2008. J Biol Chem. 283: 24047-60. PMID: 18544531
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF3ζ Antibody (H-7) | sc-271515 | 200 µg/ml | $322.00 | |||
eIF3ζ Antibody (H-7): m-IgG Fc BP-HRP Bundle | sc-529199 | 200 µg Ab; 10 µg BP | $361.00 | |||
eIF3ζ Antibody (H-7): m-IgGκ BP-HRP Bundle | sc-521966 | 200 µg Ab, 40 µg BP | $361.00 | |||
eIF3ζ Antibody (H-7): m-IgG1 BP-HRP Bundle | sc-543381 | 200 µg Ab; 20 µg BP | $361.00 | |||
eIF3ζ Antibody (H-7) AC | sc-271515 AC | 500 µg/ml, 25% agarose | $424.00 | |||
eIF3ζ Antibody (H-7) HRP | sc-271515 HRP | 200 µg/ml | $322.00 | |||
eIF3ζ Antibody (H-7) FITC | sc-271515 FITC | 200 µg/ml | $336.00 | |||
eIF3ζ Antibody (H-7) PE | sc-271515 PE | 200 µg/ml | $349.00 | |||
eIF3ζ Antibody (H-7) Alexa Fluor® 488 | sc-271515 AF488 | 200 µg/ml | $364.00 | |||
eIF3ζ Antibody (H-7) Alexa Fluor® 546 | sc-271515 AF546 | 200 µg/ml | $364.00 | |||
eIF3ζ Antibody (H-7) Alexa Fluor® 594 | sc-271515 AF594 | 200 µg/ml | $364.00 | |||
eIF3ζ Antibody (H-7) Alexa Fluor® 647 | sc-271515 AF647 | 200 µg/ml | $364.00 | |||
eIF3ζ Antibody (H-7) Alexa Fluor® 680 | sc-271515 AF680 | 200 µg/ml | $364.00 | |||
eIF3ζ Antibody (H-7) Alexa Fluor® 790 | sc-271515 AF790 | 200 µg/ml | $364.00 |